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TRACRRNA05N

Sigma-Aldrich

SygRNA Cas9 Synthetic tracrRNA

SygRNA SpCas9 tracrRNA

Synonym(s):

RNA scaffold

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

packaging

pkg of 5 nmol × Tube (HPLC)

shipped in

ambient

storage temp.

−20°C

General description

The SygRNA synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.

Application

Functional Genomics/Transgenic Applications
  • Creation of gene knockouts
  • Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes

Features and Benefits

HPLC-purified SygRNA Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.

Linkage

The SygRNA Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.

Learn more about SygRNA tracrRNA

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Physical form

Dry

Preparation Note

Resuspend SygRNA Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

Legal Information

SygRNA is a trademark of Sigma-Aldrich Co. LLC

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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