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U6253

Sigma-Aldrich

Ubiquitin from bovine erythrocytes

BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):
Ub, ATP-dependent proteolytic factor
CAS Number:
MDL number:
NACRES:
NA.32

Quality Level

biological source

bovine erythrocytes

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

mol wt

8560 Da by calculation (monoisotopic mass)
8565 Da by calculation (average mass)

extent of labeling

(Report results)

technique(s)

western blot: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... LOC(101902760)

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General description

A 76-amino acid protein (8.6 kDa) that is bound covalently by a conjugating enzyme to proteins that are targeted for degradation by 26S proteasome. Occurs in virtually all eukaryotes, including plants (thus, its name). The sequence is highly conserved; identical in animal sources from insects to humans.

Application

Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.

Packaging

5 mg in glass bottle
25 mg in poly bottle

Biochem/physiol Actions

Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.

Preparation Note

Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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