U6253

Ubiquitin from bovine erythrocytes

BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder

• Synonym(s): Ub, ATP-dependent proteolytic factor
• CAS Number: 79586-22-4
• MDL number: MFCD00082121
• NACRES: NA.32

Properties

• Quality Level: 200
• biological source: bovine erythrocytes
• product line: BioUltra
• assay: ≥98% (SDS-PAGE)
• form: essentially salt-free, lyophilized powder
• mol wt: 8560 Da by calculation (monoisotopic mass); 8565 Da by calculation (average mass)
• extent of labeling: (Report results)
• technique(s): western blot: suitable
• UniProt accession no.: A0A3Q1M4K3
• storage temp.: 2-8°C
• Gene Information: bovine ... LOC(101902760)

Description

General description

A 76-amino acid protein (8.6 kDa) that is bound covalently by a conjugating enzyme to proteins that are targeted for degradation by 26S proteasome. Occurs in virtually all eukaryotes, including plants (thus, its name). The sequence is highly conserved; identical in animal sources from insects to humans.

Application

Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.

Packaging

5 mg in glass bottle

25 mg in poly bottle

Biochem/physiol Actions

Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.

Preparation Note

Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves