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U6504

Sigma-Aldrich

Urea

suitable for electrophoresis

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NB.22

Assay

≥98%

Quality Level

form

powder or crystals

technique(s)

electrophoresis: suitable

impurities

≤0.005% Ammonia

mp

132-135 °C (lit.)

solubility

H2O: 100 mg/mL

density

1.335 g/mL at 25 °C (lit.)

cation traces

Fe: ≤5 ppm
Pb: ≤5 ppm

absorption

≤0.06 at 260 at 5 M
≤0.06 at 280 at 5 M

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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This Item
51456U5378U0631
Urea suitable for electrophoresis

Sigma-Aldrich

U6504

Urea

-
Urea BioUltra, for molecular biology, 99% (T)

Sigma-Aldrich

51456

Urea

Premium Grade
Urea powder, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

U5378

Urea

Essential+ Grade
Urea BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Sigma-Aldrich

U0631

Urea

Premium Grade
form

powder or crystals

form

powder or crystals

form

powder

form

powder, crystals or pellets

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

impurities

≤0.005% Ammonia

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.1% biuret, <0.005% formaldehyde (HCHO)

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

solubility

H2O: 100 mg/mL

solubility

H2O: 8 M at 20 °C, clear, colorless

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

solubility

H2O: 5 M at 20 °C, clear, colorless

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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