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U6504

Urea

suitable for electrophoresis

Synonym(s):

Carbamide, Carbonyldiamide

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500 G

$123.00

1 KG

$200.00

$123.00


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About This Item

Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NB.22
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Assay:
≥98%
Technique(s):
electrophoresis: suitable

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InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

SMILES string

NC(N)=O

assay

≥98%

form

powder or crystals

technique(s)

electrophoresis: suitable

impurities

≤0.005% Ammonia

Quality Level

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

mp

132-135 °C (lit.)

solubility

H2O: 100 mg/mL

density

1.335 g/mL at 25 °C (lit.)

cation traces

Fe: ≤5 ppm, Pb: ≤5 ppm

absorption

≤0.06 at 260 at 5 M, ≤0.06 at 280 at 5 M

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This Item
U063115604RDD034
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥98%

assay

98.5-101.5%

assay

99.0-100.5%, 99.0-101.0% (calc. on dry substance)

assay

≥98%

solubility

H2O: 100 mg/mL

solubility

H2O: 5 M at 20 °C, clear, colorless

solubility

H2O: soluble 480 g/L at 20 °C

solubility

-

form

powder or crystals

form

powder, crystals or pellets

form

solid

form

-

cation traces

Fe: ≤5 ppm, Pb: ≤5 ppm

cation traces

Al: ≤0.0005%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

cation traces

Fe: ≤0.5 mg/kg

cation traces

-

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Product Information Sheet

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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