U7258

Sigma-Aldrich

Anti-Ubiquilin-1 antibody produced in rabbit

enhanced validation

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-DSK2, Anti-XDRP1, Anti-UBQLN1, Anti-DA41, Anti-Protein-linking IAP to cytoskeleton 1, PLIC-1
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~62 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~0.5 mg/mL

application(s)

western blot: 0.5-1.0 μg/mL using lysates of HEK-293T cells and COS7 cells expressing human ubiquilin-1.

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... UBQLN1(29979)

General description

Ubiquilin-1 (UBQLN1) is a ubiquitin-like (UBL) protein, that contains UBL and ubiquitin-associated (UBA) domains in its N- and C-termini, respectively. UBQLN1 gene is located on the human chromosome at 9q21.32.

Specificity

Anti-Ubiquilin-1 specifically recognizes human ubiquilin-1.

Application

Anti-Ubiquilin-1 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

Ubiquilin-1 (UBQLN1) protein plays a major role in regulating the proteasomal degradation of several proteins including the presenilins PS1 and PS2. This protein is also involved in the degradation of cyclin A, γ-aminobutyric acid (GABA) receptor, and hepatitis C virus RNA-dependent RNA polymerase proteins. This protein might play a general role in neurodegenerative diseases. UBQLN1 genetic variants are associated with an increased risk of Alzheimer′s disease (AD). Downregulation of UBQLN1 gene modulates PS1 endoproteolysis. Downregulation of this gene is also involved in regulating the protein levels of nicastrin and presenilin enhancer 2 (PEN-2). It also increases the rate of β-amyloid precursor proteins (APP) maturation and trafficking through the secretory pathway associated with increased production of secretory β-amyloid precursor proteins (sAPP) and amyloid β (Aβ). UBQLN1 protein is associated with neurofibrillary tangles in AD brain and Lewy bodies in Parkinson′s disease (PD).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Unbiased screen reveals ubiquilin-1 and-2 highly associated with huntingtin inclusions
Rutherford N J, et al.
Brain Research, 1524(6), 62-73 (2013)
Relationship between ubiquilin-1 and BACE1 in human Alzheimer's disease and APdE9 transgenic mouse brain and cell-based models
Natunen T, et al.
Neurobiology of Disease, 85(6), 187-205 (2016)
Identification of ubiquilin, a novel presenilin interactor that increases presenilin protein accumulation
Mah A L, et al.
The Journal of cell biology, 151(4), 847-862 (2000)
Gender-specific association of ATP-binding cassette transporter 1 (ABCA1) polymorphisms with the risk of late-onset Alzheimer's disease
Sundar P D, et al.
Neurobiology of Aging, 28(6), 856-862 (2007)
Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin
Massey L K, et al.
The Biochemical Journal, 391(3), 513-525 (2005)
Articles
Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions.
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