U8757

Sigma-Aldrich

Ubiquitin-Carrier Protein H5b human

≥95% (SDS-PAGE), solution

Synonym(s):
UbcH5b
MDL number:

biological source

human

recombinant

expressed in E. coli (GST-tagged)

assay

≥95% (SDS-PAGE)

form

solution

mol wt

47 kDa

application(s)

ligand binding assay: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBE2D2(7322)

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

Ubiquitin-Carrier Protein H5c human was used to study the role of ubiquitin-proteasome system in pathogenesis of human diseases.

Biochem/physiol Actions

Mediates the degradation of a myriad of short-lived regulatory proteins including the tumor suppressor p53 in the presence of E6/E6-AP or MDM2, the c-Fos transcription factor, and the signal-induced ubiquitination of IκBα, UBC5 is an essential gene in yeast.
Ubiquitin-Carrier Protein H5b human also referred to as UbcH5b is a recombinant protein that enhances the activation of NF-κB via ubiquitination as well as facilitates in proteasome-mediated degradation of its inhibitor κBα. It also assists in regulating p53 levels by ubiquitination.

Physical form

Solution in 50 mM HEPES, pH 8.0, 100 mM NaCl, 10% glycerol

Certificate of Analysis

Certificate of Origin

The ubiquitin-proteasome proteolytic pathway.
A Ciechanover
Cell, 79(1), 13-21 (1994-10-07)
Mark K Saville et al.
The Journal of biological chemistry, 279(40), 42169-42181 (2004-07-29)
p53 levels are regulated by ubiquitination and 26 S proteasome-mediated degradation. p53 is a substrate for the E3 ligase Mdm2, however, the ubiquitin-conjugating enzymes (E2s) involved in p53 ubiquitination in intact cells have not been defined previously. To investigate the...
J P Jensen et al.
The Journal of biological chemistry, 270(51), 30408-30414 (1995-12-22)
Two very closely related human E2 ubiquitin conjugating enzymes, UbfH5B and UbcH5C, have been identified. These enzymes are products of distinct genes and are 88-89% identical in amino acid sequence to the recently described human E2, UbcH5 (now designated UbcH5A)...
H Gonen et al.
The Journal of biological chemistry, 274(21), 14823-14830 (1999-05-18)
The last step in the activation of the transcription factor NF-kappaB is signal-induced, ubiquitin- and proteasome-mediated degradation of the inhibitor IkappaBalpha. Although most of the components involved in the activation and degradation pathways have been identified, the ubiquitin carrier proteins...

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