The optimal pH for this enzyme ranges from 3.3 to 5.5, and the optimal temperature is between 40 to 50 °C. For stock solution preparation, 30 mM PBS pH 5.0 may be used for reconstitution.
V2010
Viscozyme® L
cellulolytic enzyme mixture
Synonym(s):
Cell Wall Degrading Enzyme Complex from Aspergillus sp., Lysing Enzyme from Aspergillus sp.
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biological source
fermentation/recombinant
Quality Level
form
liquid
enzyme activity
≥100 FBGU/g
technique(s)
protein extraction: suitable
density
~1.2 g/mL at 25 °C (lit.)
suitability
suitable for cell lysis
application(s)
cell analysis
storage temp.
2-8°C
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This Item | SAE0050 | P4032 | G5160 |
|---|---|---|---|
| biological source fermentation/recombinant | biological source - | biological source Aspergillus sp. (A. melleus) | biological source - |
| technique(s) protein extraction: suitable | technique(s) - | technique(s) - | technique(s) - |
| suitability suitable for cell lysis | suitability - | suitability - | suitability - |
| form liquid | form - | form solid | form solid |
| application(s) cell analysis | application(s) - | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
General description
Viscozyme® L is a lignocellulose-specialized multi-enzymatic complex derived from Aspergillus aculeatus.[1] It contains a wide range of carbohydrases, including arabanase, cellulase, β-glucanase, hemicellulase, and xylanase.
Application
Viscozyme® L has been used:
- in the extraction of polyphenol from grapefruit and unripe apples.
- to study the effect of its supplementation on nutritive value and silage characteristics of sunflower silage.
- for simulation of the fermentation process during in vitro digestibility of former food products (FFPs).
- for enzymatic treatment, to study its effects on the antioxidant properties of wheat bran.[2]
Biochem/physiol Actions
Viscozyme L aids in the release of oil bodies and proteins from cells by degrading the hemicellulose, cellulose, and pectin molecules, and by breaking the cell wall components.
Features and Benefits
- Not a GMO and doesn′t contain GMOs.
- Made by fermentation of a microorganism.
- Improves process through faster, more efficient oil/water separation.
Legal Information
A product of Novozyme Corp.
Viscozyme is a registered trademark of Novozymes Corp.
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Tackling the Challenge of Extracting Microplastics from Soils: A Protocol to Purify Soil Samples for Spectroscopic Analysis
Moller J N, et al.
Environmental Chemistry (2021)
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New biotechnology, 62, 60-67 (2021-02-01)
Polyhydroxyalkanoates (PHAs) are polyesters of significant interest due to their biodegradability and properties similar to petroleum-derived plastics, as well as the fact that they can be produced from renewable sources such as by-product streams. In this study, brewer's spent grain
Effects of Solid-State Enzymatic Treatments on the Antioxidant Properties of Wheat Bran
Moore J, et al.
Journal of Agricultural and Food Chemistry, 54 (24), 9032?9045-9032?9045 (2006)
Estelle Couradeau et al.
PloS one, 6(12), e28767-e28767 (2011-12-24)
The geomicrobiology of crater lake microbialites remains largely unknown despite their evolutionary interest due to their resemblance to some Archaean analogs in the dominance of in situ carbonate precipitation over accretion. Here, we studied the diversity of archaea, bacteria and
Lovedeep Kaur et al.
Foods (Basel, Switzerland), 10(2) (2021-02-10)
Being widely abundant, grass proteins could be a novel source of plant proteins for human foods. In this study, ryegrass proteins extracted using two different approaches-chemical and enzymatic extraction, were characterised for their physico-chemical and in vitro digestion properties. A
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What is the optimum pH and Temperature of this enzyme?
1 answer-
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What is the concentration I should use?
1 answer-
For the reduction of beta-glucans and viscosity, a dosage of 0.02-0.1% of grist weight is recommended. For other applications, a general recommendation is 0.05-0.1%.
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What does the hemicellulases of this product contain? Can it degrade mannan or galactan?
1 answer-
The key enzyme activity is endo-beta-glucanase, which hydrolyzes (1,3)- or (1,4)-linkages in beta-D-glucans. This product contains side activities of xylanase, cellulase, and hemicellulose. Mannan or galactan degradation is not expected.
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What's the cas number of Viscozyme L?
1 answer-
A CAS Registry Number is assigned to specific compounds. This product is a blend of beta-glucanases, pectinases, hemicellulases and xylanases and is not assigned a CAS number.
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What enzymes does this product contain exactly? And does the ability to degrade pectin mean this product contains pectinase? And what type of hemicellulase does this product contain?
1 answer-
This product is a blend of beta-glucanases, pectinases, hemicellulases, and xylanases.
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How many units of enzyme in this 50ml?
1 answer-
The unit value of this product is lot specific and reported on the Certificate of Analysis. A survey of the most recent lots shows and average activity of 108 units per gram and a density of 1.21 g/ml. Please see the link below to access the lot specific Certificate:
https://www.sigmaaldrich.com/searchHelpful?
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