V2258

Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone LN-6, ascites fluid

MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LN-6, monoclonal

contains

15 mM sodium azide

species reactivity

rabbit, mouse, rat, feline, sheep, human, pig, bovine

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable using cultured cells
western blot: suitable

isotype

IgM

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)

General description

Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.
Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells.

Specificity

Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.

Immunogen

human thymic nuclear extract

Application

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .
Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling.

Biochem/physiol Actions

Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Role of vimentin in early stages of neuritogenesis in cultured hippocampal neurons
Boyne LJ, et al.
International Journal of Developmental Neuroscience, 14(6), 739-748 (1996)
M J Gibson et al.
Endocrinology, 141(9), 3065-3071 (2000-08-31)
Axons of GnRH neurons terminate at the median eminence in the medial basal hypothalamus (MBH) of the brain early in development. Similarly, GnRH neurons in grafts of preoptic area (POA) tissue within the third ventricle of hypogonadal mice preferentially innervate...
Vimentin is a crucial target for anti-metastasis therapy of nasopharyngeal carcinoma
Wang W, et al.
Molecular and Cellular Biochemistry, 438(1-2), 47-57 (2018)
Margit A Huber et al.
The Journal of clinical investigation, 114(4), 569-581 (2004-08-18)
The transcription factor NF-kappaB is activated in a range of human cancers and is thought to promote tumorigenesis, mainly due to its ability to protect transformed cells from apoptosis. To investigate the role of NF-kappaB in epithelial plasticity and metastasis...
Christopher M Westerkamp et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 289(4), R1223-R1231 (2005-06-18)
Because optimal overload-induced skeletal muscle hypertrophy requires ANG II, we aimed to determine the effects of blocking ANG II production [via angiotensin-converting enzyme (ACE) inhibition] on potential mediators of hypertrophy in overloaded skeletal muscle, namely, myonuclear addition and fibroblast content....
Articles
In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
Read More
Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.