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V2258

Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone LN-6, ascites fluid

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Synonym(s):
Anti-F5H288
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LN-6, monoclonal

contains

15 mM sodium azide

species reactivity

pig, mouse, sheep, human, bovine, rabbit, feline, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable using cultured cells
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)

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This Item
V5255V6389SAB4200761
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

LN-6, monoclonal

clone

VIM-13.2, monoclonal

clone

V9, monoclonal

clone

LN-6, monoclonal

species reactivity

pig, mouse, sheep, human, bovine, rabbit, feline, rat

species reactivity

chicken, rat, human, rabbit, frog, mouse

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human

species reactivity

rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections, immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable using cultured cells, western blot: suitable

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts, western blot: 1:500 using human fibroblasts cell extract

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue, microarray: suitable, western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68

technique(s)

flow cytometry: suitable, immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast Hs68 cell line., immunofluorescence: 1-2 μg/mL using human foreskin fibroblast Hs68 cell line., immunohistochemistry: 5-10 μg/mL using Human breast carcinoma sections., immunoprecipitation (IP): suitable

General description

Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells.
Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.

Specificity

Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.

Immunogen

human thymic nuclear extract

Application

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .
Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling.

Biochem/physiol Actions

Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair
Prignano F, et al.
Dermatologic Therapy, 22, S8-S15 (2009)
Alternatively spliced lysyl oxidase-like 4 isoforms have a pro-metastatic role in cancer
Sebban S, et al.
Clinical & Experimental Metastasis, 30(1), 103-117 (2013)
Role of vimentin in early stages of neuritogenesis in cultured hippocampal neurons
Boyne LJ, et al.
International Journal of Developmental Neuroscience, 14(6), 739-748 (1996)
Vimentin is a crucial target for anti-metastasis therapy of nasopharyngeal carcinoma
Wang W, et al.
Molecular and Cellular Biochemistry, 438(1-2), 47-57 (2018)
M J Gibson et al.
Endocrinology, 141(9), 3065-3071 (2000-08-31)
Axons of GnRH neurons terminate at the median eminence in the medial basal hypothalamus (MBH) of the brain early in development. Similarly, GnRH neurons in grafts of preoptic area (POA) tissue within the third ventricle of hypogonadal mice preferentially innervate

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