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V3638

Sigma-Aldrich

Vascular Endothelial Growth Factor 164 from rat

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

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Synonym(s):
VEGF 164, VEGF-164, VEGF164, Vasculotropin
MDL number:
NACRES:
NA.32

biological source

rat

Quality Level

recombinant

expressed in NSO cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1.08 ng/mL ED50

mol wt

dimer 50 kDa by SDS-PAGE
monomer 25 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
SRP4365V7259P5739
VEGF165 from rat recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

Sigma-Aldrich

SRP4365

VEGF165 from rat

recombinant

expressed in NSO cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

assay

>95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

>97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

powder

form

lyophilized powder

potency

1.08 ng/mL ED50

potency

-

potency

1-10 ng/mL ED50/EC50

potency

-

mol wt

dimer 50 kDa by SDS-PAGE, monomer 25 kDa by SDS-PAGE

mol wt

~38.4 kDa

mol wt

protein 38.2 kDa (as homodimer)

mol wt

~38 kDa by SDS-PAGE

Application

Vascular Endothelial Growth Factor 164 (VEGF 164) from rat was used for cardiomyocytes culture and Ca2+ transients′ measurement. It was used as a medium M-199 supplement utilized in in vitro incubation for examining the capability of CTS to limit the impact of biologically relevant molecules, in particular epidermal growth factor (EGF), basic fibroblast growth factor (FGF2), and vascular endothelial growth factor A (VEGFA) in rat epididymis segment 1 and 2.

Biochem/physiol Actions

Vascular Endothelial Growth Factor 164 also referred to as VEGF 164 or Vasculotropin is a growth factor that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF 164 can enhance collagen and fibronectin expression in mesangial cells which in turn stimulates TGF-β1-induced extracellular matrix. It also plays a pivotal role in progressive glomerular fibrosis.
Mitogen for endothelial cells, enhances vascular permeability, activates, and chemoattracts monocytes and is a procoagulant.

Other Notes

164 amino acid variant of rat VEGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Mild traumatic brain injury (mTBI) is a common and unmet clinical issue, with limited treatments available to improve recovery. The cerebrovascular system is vital to provide oxygen and nutrition to the brain, and a growing body of research indicates that
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The Journal of biological chemistry, 279(32), 33213-33219 (2004-05-15)
Transforming growth factor-beta1 (TGF-beta1) is a potent inducer of extracellular matrix synthesis leading to progressive glomerular fibrosis. The intracellular signaling mechanisms involved in this process remain incompletely understood. The p38 mitogen-activated protein kinase (MAPK) is a major stress signal transducing
G Neufeld et al.
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Articles

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell migration, proliferation, tube formation, and survival. In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization.

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