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V4512

Sigma-Aldrich

Vascular Endothelial Growth Factor from mouse

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
mVEGF, VEGF
MDL number:
NACRES:
NA.77

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

powder

potency

≤10 ng/mL

quality

endotoxin tested

mol wt

protein 39 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... VEGFA(22339)

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SRP6430V7259SRP4364
VEGF164 from mouse recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE)

Sigma-Aldrich

SRP6430

VEGF164 from mouse

VEGF165 from mouse recombinant, expressed in E. coli

Sigma-Aldrich

SRP4364

VEGF165 from mouse

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98%

form

powder

form

lyophilized

form

powder

form

lyophilized

potency

≤10 ng/mL

potency

-

potency

1-10 ng/mL ED50/EC50

potency

1.0-5 ng/mL

mol wt

protein 39 kDa

mol wt

calculated mol wt 19.3 kDa (no tag), observed mol wt 23-25 kDa (DTT-reduced. Protein migrates due to glycosylation. Ala 27 is the predicted N-terminal.)

mol wt

protein 38.2 kDa (as homodimer)

mol wt

~39.0 kDa

General description

Vascular endothelial growth factor is also known as VPF, VEGF and MVCD1. It is secreted from endothelial cells and pericytes in response to hypoxia. It helps in inducing angiogenesis and microvascular hyperpermeability. VEGF levels are associated with vasculitic neuropathy and may be used to predict this disease. It may also act as a marker in the development and progression of early precancerous lesions of oesophagus.

Application

Vascular endothelial growth factor (VEGF) supports development of new blood vessels during embryonic development and after vascular injury.Vascular endothelial growth factor from mouse was used for ELISA and in matrigel plugs (matrigel was supplemented with VEGF).

Other Notes

Homodimeric protein consisting of two 165 amino acid polypeptide chains of mouse VEGF.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured by the ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells (HUVEC) and bovine aortic endothelial cells.

Storage Class Code

11;13 - Combustible Solids;Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Vascular Endothelial Growth Factors (VEGF)

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell migration, proliferation, tube formation, and survival. In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization.

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