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V5255

Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone VIM-13.2, ascites fluid

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Synonym(s):
Anti-F5H288
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

VIM-13.2, monoclonal

species reactivity

chicken, rat, human, rabbit, frog, mouse

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
western blot: 1:500 using human fibroblasts cell extract

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)

Related Categories

General description

Vimentin is a 53 kD type III intermediate filaments expressed in breast myoepithelial cells, osteocytes, langerhans cells of the skin, schwann cells and astrocytes. Monoclonal anti-vimentin antibody can be used in immunofluorescent labeling of frozen tissue sections to localize vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes. It can also be used in western blotting. Mouse anti-vimentin antibody reacts specifically with vimentin. This product has also shown best reactivity with cultured chicken fibroblasts and can also react with human and mouse cells or tissues.The protein monomers consist of α-helical rod, flanked by head and tail domains. The VIM gene is mapped to human chromosome 10p13.

Immunogen

human foreskin fibroblasts

Application

Monoclonal anti-vimentin antibody can be used in the following:
  • Immunocytochemical localization of vimentin in normal and pathological tissue of mesenchymal origin.
  • It can also be used in immunoblotting
  • Immunofluorescence
  • Further, anti-vimentin antibody can be used to distinguish tumors and metastatic lesions derived from sarcomas, lymphomas, and melanomas.

Biochem/physiol Actions

Vimentin has a crucial role in maintaining cell shape and motility. It also provides mechanical stress tolerance for mesenchymal cells. Vimentin induces fibroblast motility and promotes wound healing process. Vimentin filaments form a part of cytoskeleton filament system and therefore, support and secure the cell organelles and also maintain cellular integrity. Vimentin is associated with the process of initiation, progression, metastasis and epithelial-mesenchymal transition of cancer cells, thereby contributing to tumorigenesis. Upregulation of this gene is observed in various cancers including prostate cancer, gastrointestinal tumors, lung cancer, breast cancer, CNS tumors and malignant melanoma.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200716 Anti-Vimentin antibody, Mouse monoclonal
clone VIM-13.2, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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