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Anti-Vimentin antibody, Mouse monoclonal

clone V9, purified from hybridoma cell culture

Anti-Vimentin antibody, Mouse monoclonal

biological source


Quality Level



antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies


V9, monoclonal


buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human


antibody small pack of 25 μL


~1 mg/mL


flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue
microarray: suitable
western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... VIM(7431)
rat ... Vim(81818)

General description

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the V9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with vimentin purified from pig eye lens.
Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α--helical amino- terminal “head” and carboxy-terminal “tail” domain.


The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.


pig eye lens vimentin.


Monoclonal Anti-Vimentin antibody produced in mouse has been used in immunohistochemical analysis and western blot assay.
Monoclonal Anti-Vimentin antibody produced in mouse has been used in:
  • immunofluorescence
  • immunohistochemistry
  • immunocytochemistry
  • immunoblotting
  • flow cytometry.


25, 100, 200 μL

Biochem/physiol Actions

Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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