V6629

Vitamin B₁₂

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%

• Synonym(s): α-(5,6-Dimethylbenzimidazolyl)cyanocobamide, CN-Cbl, Cyanocob(III)alamin, Cyanocobalamin
• Empirical Formula (Hill Notation): C₆₃H₈₈CoN₁₄O₁₄P
• CAS Number: 68-19-9
• Molecular Weight: 1355.37
• PubChem Substance ID: 24900763
• eCl@ss: 34058004
• UNSPSC Code: 12352205
• NACRES: NA.75
• EC Number: 200-680-0
• Beilstein/REAXYS Number: 4122889

Properties

• Product Name: Vitamin B₁₂, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%
• InChI key: RMRCNWBMXRMIRW-QJRSUKKJSA-L
• InChI: 1S/C62H90N13O14P.CN.Co/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;1-2;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);;/q;;+2/p-2/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57?,59-,60+,61+,62+;;/m1../s1
• SMILES string: C/C(C1=N[C@]([C@]([C@H](CC(N)=O)[C@@]/2(C)CCC(NC[C@@H](OP([O-])(O[C@H]3[C@@H](O)[C@@H](N4C=[N]5C6=C4C=C(C)C(C)=C6)O[C@@H]3CO)=O)C)=O)([H])N([Co+]5C#N)C2=C(C)/C([C@@H](CCC(N)=O)C/7(C)C)=NC7=C/8)(C)[C@@](C)(CC(N)=O)[C@@H]1CCC(N)=O)=C9N=C8[C@@H](CCC(N)=O)[C@
• biological source: synthetic ((organic))
• product line: BioReagent
• assay: ≥98%
• form: powder
• mol wt: M_w 1355.37 g/mol
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• color: red to brown
• pH: 6
• solubility: water: soluble 50 mg/mL, dark red
• storage temp.: 2-8°C
• Quality Level: 200

Description

Application

Vitamin B12 has been used:

• as a component of Erdschreiber′s medium[1]
• as a supplement of Dulbecco′s modified Eagle′s medium used for the isolation of ventricular myocytes from neonatal and adult rats[2]
• orally administered to male quails to study its role in reducing the negative effects of sodium selenite intoxication, in vivo[3]
• as a supplement in DMEM/ medium 199 (M199) medium for differentiation of human myoblasts.[4]

Biochem/physiol Actions

Vitamin B12 is metabolized to its active form, methylcobalamin.[3] Hindered vitamin B12 uptake is observed in pernicious anemia.[5] Vitamin B12 is essential for erythroblast development. Pregnancy reduces the levels of vitamin B12.[6] Aging reduces vitamin B12 absorption.[7] It works as a coenzyme in fat, carbohydrate and protein metabolism.[3] It is required for growth, genetic stability and survival of cells in vitro. Functions to support one-carbon metabolism. Present in many classical and serum-free formulations.

General description

Cyanocobalamin (B12) is a water soluble vitamin, commonly called as cobalamins. Dairy products, meat and eggs are the major sources of this vitamin.[8]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)