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V6630

Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone V9, ascites fluid

Synonym(s):
Monoclonal Anti-Vimentin
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

V9, monoclonal

mol wt

antigen ~58 kDa

contains

15 mM sodium azide

species reactivity

chicken, rat, bovine, feline, hamster, gerbil, horse, canine, rabbit, monkey, pig, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue
western blot: 1:200 using human fibroblasts HS-68

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

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General description

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved a-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

Specificity

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Immunogen

pig eye lens vimentin.

Application

Monoclonal Anti-Vimentin antibody has been used in western blotting and immunocytochemistry.
Monoclonal Anti-Vimentin antibody produced in mouse has been used:
  • in immunofluorescence Analysis
  • in western blot
  • anti-vimentin antibody is injected into mature oocytes before embryo manipulations to bind endogenous vimentin (VIM)
  • in immunocytochemistry
Monoclonal Anti-Vimentin antibody produced in mouse has been used as a primary antibody to localize vimentin by immunohistochemistry (IHC) and for immunoprecipitation of vimentin.

Biochem/physiol Actions

Vimentin is the major cytoskeletal component in immature glia. It is an intermediate filament protein and serves as a substrate for several caspases. The cleavage of this protein by caspase dismantles intermediate filaments and promotes apoptosis.
Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.
Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic spread of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
V6389 Anti-Vimentin antibody, Mouse monoclonal
clone V9, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the epitope sequence for product V6630, Monoclonal Anti-Vimentin antibody produced in mouse?

    Product V6630, Monoclonal Anti-Vimentin antibody, is a licensed antibody. The development and specification of this clone are specified in: Osborn, M., et al., Europ. J. Cell Biol., 34 137-143 (1984). In this paper the immunogen is porcine vimentin.

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    Ask a Scientist here.

D Dahl et al.
European journal of cell biology, 24(2), 191-196 (1981-06-01)
Comparison of cytoskeletal preparations obtained from newborn and adult rat brain showed similar patterns on SDS-PAGE. However, coelectrophoresis of the newborn and adult preparations revealed distinct differences in the mobility of 2 major bands in the molecular weight range of
The gene encoding human vimentin is located on the short arm of chromosome 10.
Ferrari S , et al.
American Journal of Human Genetics, 41(4), 616?626-616?626 (1987)
Androgen-independent prostate cancer DU145 cells suppress androgen-dependent growth of prostate stromal cells through production of inhibitory factors for androgen responsiveness.
Minamiguchi K, et al.
Biochemical and Biophysical Research Communications, 306(3), 629-636 (2003)
Vimentin modulates infectious internalisation of HPV16 pseudovirions.
Schafer G, et al.
Journal of Virology, 91(16), e00307-e00317 (2017)
Kei Miyamoto et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(17), 7040-7045 (2011-04-13)
Nuclear reprogramming of differentiated cells can be induced by oocyte factors. Despite numerous attempts, these factors and mechanisms responsible for successful reprogramming remain elusive. Here, we identify one such factor, necessary for the development of nuclear transfer embryos, using porcine

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