V7259

Sigma-Aldrich

Vascular Endothelial Growth Factor human

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
VEGF
CAS Number:
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

powder

potency

1-10 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

protein 38.2 kDa (as homodimer)

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤0.1 EU/μg

color

white

solubility

water: soluble

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

General description

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

Application

Vascular Endothelial Growth Factor (VEGF) has been used for-
  • the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
  • inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Biochem/physiol Actions

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Physical properties

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Physical form

Lyophilized from a sterile filtered buffered solution with no additives.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Behrous Davani et al.
Stem cells (Dayton, Ohio), 25(12), 3215-3222 (2007-09-29)
Islet transplantation offers improved glucose homeostasis in diabetic patients, but transplantation of islets is limited by the supply of donor pancreases. Undifferentiated precursors hold promise for cell therapy because they can expand before differentiation to produce a large supply of...
Michael Bressan et al.
Development (Cambridge, England), 141(21), 4149-4157 (2014-10-03)
Efficient blood flow depends on two developmental processes that occur within the atrioventricular junction (AVJ) of the heart: conduction delay, which entrains sequential chamber contraction; and valve formation, which prevents retrograde fluid movement. Defects in either result in severe congenital...
The biology of vascular endothelial growth factor.
N Ferrara et al.
Endocrine reviews, 18(1), 4-25 (1997-02-01)
Kazuhide Hayakawa et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(29), 10666-10670 (2011-07-22)
Originally identified as an angiogenic factor, vascular endothelial growth factor (VEGF-A) is now known to play multiple roles in the CNS, including the direct regulation of neuronal and astrocytic functions. Here, we ask whether VEGF-A can also have a novel...
Samara S Souza et al.
PloS one, 15(11), e0241442-e0241442 (2020-11-05)
Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation...
Articles
Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell migration, proliferation, tube formation, and survival. In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization.
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