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V7881

Sigma-Aldrich

Monoclonal Anti-Vitronectin antibody produced in mouse

clone VIT-2, ascites fluid

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Synonym(s):
Anti-V75
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

VIT-2, monoclonal

mol wt

antigen 65-75 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

indirect ELISA: suitable
indirect immunofluorescence: suitable using human cultured fibroblasts
microarray: suitable
western blot: 1:2,500 using a denatured and reduced preparation of purified human plasma vitronectin

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VTN(7448)

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V4505V9131F6140
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

clone

VIT-2, monoclonal

clone

VIN-11-5, monoclonal

clone

hVIN-1, monoclonal

clone

FN-3E2, monoclonal

mol wt

antigen 65-75 kDa

mol wt

antigen 116 kDa

mol wt

antigen 116 kDa

mol wt

antigen 240 kDa

species reactivity

human

species reactivity

bovine, human, chicken, mouse

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

species reactivity

mouse, human, chicken

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General description

Monoclonal Anti-Vitronectin (mouse IgM isotype) is derived from the VIT-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human plasma vitronectin. Vitronectin is also referred as serum-spreading factor, S-protein of complement or epibolin. This monomeric acidic protein is one of the major multifunctional cell-adhesive glycoproteins in mammalian plasma and serum. This protein is usually seen as a mixture of 75 kDa and 65 kDa polypeptides. Human plasma and serum contain 0.1-0.4 mg/ml of vitronectin which is synthesized in the liver. It is also present in amniotic fluid and urine.

Specificity

By immunoblotting, the product shows no cross-reactivity with fibronectin, laminin, merosin, collagen type IV or chondroitin sulfate types A, B and C.

Immunogen

human plasma vitronectin

Application

Monoclonal Anti-Vitronectin antibody produced in mouse has been used in:
  • immunocytochemisry
  • western blotting
  • immunoprecipitation
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b-9 complex. Vitronectin protects thrombin from inactivation by antithrombin III in the presence of heparin, binds and stabilizes the activity of plasminogen activator inhibitor and mediates many other physiological functions.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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P Dufourcq et al.
Arteriosclerosis, thrombosis, and vascular biology, 18(2), 168-176 (1998-03-04)
Vitronectin (VN) is a plasma glycoprotein that promotes cell attachment and induces migration of human smooth muscle cells (SMCs) in culture. VN has been observed to accumulate in human atherosclerotic plaques, although its origin and role in atherosclerosis are not
Ovarian cancer ascites-derived vitronectin and fibronectin: Combined purification, molecular features and effects on cell response
Carduner L, et al.
Biochim. Biophys. Acta Gen. Subj., 1830(10), 4885-4897 (2013)
Novel purification of vitronectin from human plasma by heparin affinity chromatography
Yatohgo T, et al.
Cell Structure and Function, 13(4), 281-292 (1988)
L Bello et al.
Neurosurgery, 49(2), 380-389 (2001-08-16)
This study analyzed the expression of integrins alpha(v)beta3 and alpha(v)beta5 in glioma tissue and focused on the periphery of high-grade gliomas. The analysis was performed with Western blot, immunohistochemistry, and immunofluorescence, by use of two monoclonal antibodies able to recognize
Hepatocyte Growth Factor Regulates E Box-Dependent Plasminogen Activator Inhibitor Type 1 Gene Expression in HepG2 Liver Cells
Imagawa S, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology (2006)

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