VRE100
Viral RNA Extraction Buffer
One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva
Synonym(s):
For use with Reverse Transcription-qPCR, Viral Lysis Solution for RT-qPCR, Viral RNA Extraction
About This Item
Recommended Products
grade
Molecular Biology
Quality Level
form
liquid
concentration
4X
technique(s)
LAMP: suitable
PCR: suitable
RT-PCR: suitable
qPCR: suitable
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Application
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
- Quantitative RT-PCR ReadyMix™ (Sigma-Aldrich, catalog QR0200)
- KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
- KiCqStart® One-Step Probe RT-qPCR ReadyMix™ (Sigma-Aldrich, catalog KCQS07)
- TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
Features and Benefits
- Extracted viral RNA can be used for detection without cleanup
- Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and loop mediated isothermal amplification(LAMP) assays
- Ideal for enveloped RNA viruses in saliva, saline, Amies medium, and viral transport medium
- Highly sensitive and faster than other leading methods, lysis by Proteinase K and heating
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.
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