Viral inactivation after incubation with VRE100 has not been tested.
VRE100
Viral RNA Extraction Buffer
One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva
Synonym(s):
For use with Reverse Transcription-qPCR, Viral Lysis Solution for RT-qPCR, Viral RNA Extraction
Skip To
grade
Molecular Biology
Quality Level
form
liquid
concentration
4X
technique(s)
LAMP: suitable
PCR: suitable
RT-PCR: suitable
qPCR: suitable
shipped in
wet ice
storage temp.
−20°C
1 of 4
This Item | RNV100 | 3095 | KCQS09 |
|---|---|---|---|
| grade Molecular Biology | grade - | grade - | grade - |
| technique(s) LAMP: suitable, RT-PCR: suitable, qPCR: suitable | technique(s) - | technique(s) DNA amplification: suitable | technique(s) RT-qPCR: suitable |
| concentration 4X | concentration - | concentration - | concentration - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level - |
| form liquid | form - | form - | form liquid |
| storage temp. −20°C | storage temp. 15-25°C | storage temp. - | storage temp. −20°C |
General description
The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.
Application
The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX.
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
- Quantitative RT-PCR ReadyMix™ (Sigma-Aldrich, catalog QR0200)
- KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
- KiCqStart® One-Step Probe RT-qPCR ReadyMix™ (Sigma-Aldrich, catalog KCQS07)
- TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
Features and Benefits
- Extracted viral RNA can be used for detection without cleanup
- Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and loop mediated isothermal amplification(LAMP) assays
- Ideal for enveloped RNA viruses in saliva, saline, Amies medium, and viral transport medium
- Highly sensitive and faster than other leading methods, lysis by Proteinase K and heating
Legal Information
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.
Related Content
Instructions
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Is VRE100 capable of inactivating virus?
1 answer-
Helpful?
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Can samples lysed with VRE100 be stored for future use?
1 answer-
Yes, treated samples can be stored at -20 °C until ready for RNA detection. Multiple freeze-thaws are not recommended.
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Which viral transport media can be used with VRE100?
1 answer-
VRE100 has been shown to be compatible with saline, including phosphate-buffered saline (PBS) and Hank’s Buffered Saline Solution (HBSS), as well as with standard viral transport medium containing 2% FBS. Transport media containing guanidine are not recommended for use with VRE100 as guanidine will interfere with direct nucleic acid detection techniques, such as RT-qPCR. VRE100 is also compatible with undiluted saliva.
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Can VRE100 be used for nasal swabs?
1 answer-
VRE100 has not been tested for use with nasal swabs, and is a Research Use Only product.
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What ratio of VRE100 to sample is recommended?
1 answer-
VRE100 was developed as a 4x lysis solution, such that 1 part buffer is added to 3 volume equivalents of sample. However, improved detection sensitivity may be achieved in certain samples by increasing the amount of VRE100 used for sample lysis.
Helpful?
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Is there any reaction stoppage step required?
1 answer-
No reaction stoppage step is required. Treated samples can be kept at room temperature for up to 30 min. If analyzing RNA within 1-2 hours samples can be kept on ice, otherwise samples should be frozen until ready for RNA detection.
Helpful?
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Which viruses can be detected using VRE100?
1 answer-
RNA from SARS-CoV-2, Influenza A and B, and Respiratory Syncytial virus (RSV) have been detected using VRE100 to lyse crosslink-inactivated viral particles in Viral Transport Medium.
Helpful?
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