W0138

Western Blocker^™ Solution

for HRP detection systems

• Synonym(s): Western blot blocking solution, blocking buffer
• UNSPSC Code: 41105329
• NACRES: NA.25

Properties

• Quality Level: 200
• shelf life: ≥2 yr
• storage temp.: 2-8°C

Description

General description

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Application

Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes.[1][2][3] It has also been used for dilution of antibodies in western blot.[4]

Features and Benefits

• Does not inhibit weak HRP signals, as many milk-based blockers do.
• Can be used both as a blocking component and as a probing component.
• Supplied ready-to-use; no preparative step is required.

Legal Information

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable