Williams′ Medium E

With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture


Quality Level






cell culture | mammalian: suitable


endotoxin, tested


phenol red: no
glucose: 2.0 g/L (Dextro)
sodium pyruvate: 0.025 g/L
L-glutamine: no
NaHCO3: 2.2 g/L

shipped in


storage temp.


General description

Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.


Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.


Supplement with 0.292 g/L L-glutamine.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Dariusz Ratman et al.
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Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on...
Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2...
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Stem cells translational medicine, 6(3), 851-863 (2017-03-16)
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Ezgi Eyluel Bankoglu et al.
PloS one, 11(11), e0166956-e0166956 (2016-11-29)
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in...

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