WTA1

Sigma-Aldrich

TransPlex® Whole Transcriptome Amplification Kit

DNA polymerase separate.

NACRES:
NA.55

Quality Level

application(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

General description

TransPlex, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Application

TransPlex®  Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA.
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Features and Benefits

  • Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
  • Only 5 ng of starting material required to produce a highly representative library from total RNA
  • Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Principle

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.

Legal Information

TransPlex is a registered trademark of Rubicon Genomics, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS

  • W4502Water, Nuclease-Free Water, for Molecular Biology

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?

    The dNTP Mix (D6193) is supplied as 10 mM each.

  4. Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?

    Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.

  5. What is the difference between WTA and WGA?   I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.

    The starting material for WTA is RNA, whereas for WGA it is genomic DNA.  After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.

  6. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?

    Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.

  7. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?

    Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Articles
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
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Protocols
TransPlex WTA amplification product is suitable as microarray target for expression analysis on the Affymetrix platform, and can be incorporated into existing Affymetrix workflows
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Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
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Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
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Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing Agilent workflows
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Related Content
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
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