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WTA1

TransPlex® Whole Transcriptome Amplification Kit

DNA polymerase separate.

Synonym(s):

Transcriptome Amplification Kit

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50 reactions
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$3,470.00

About This Item

NACRES:
NA.55
UNSPSC Code:
41121800

$3,470.00

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Quality Level

200

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

General description

TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Application

TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.[1]
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Biochem/physiol Actions

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.

Features and Benefits

  • Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
  • Only 5 ng of starting material required to produce a highly representative library from total RNA
  • Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Legal Information

TransPlex is a registered trademark of Rubicon Genomics, Inc.

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TransPlex® Whole Transcriptome Amplification Kit DNA polymerase separate.

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SEQXE

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technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

input

purified DNA

input

purified RNA

input

purified RNA

input

purified DNA

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

dilution

(WTA)

dilution

(WTA)

dilution

(WTA)

dilution

(WGA)

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • W4502
    Water, Nuclease-Free Water, for Molecular Biology
    SDS
  • D7295
    Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent
    SDS

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Storage Class

12 - Non Combustible Liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCF9667
SLCC3063
SLBZ6319
SLBQ6830V
SLBP1558V

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Protocols

Integration of Sigma® TransPlex® with Agilent Microarray

TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

Related Content

Whole Transcriptome Amplification FAQs

Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

Product Information Sheet - WTA1

Instructions

Munehiro Okamoto et al.
Scientific reports, 5, 8850-8850 (2015-03-07)
We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously
Sa Xiao et al.
Virus research, 145(1), 80-91 (2009-06-23)
The complete genome sequence of avian paramyxovirus serotype 7 (APMV-7) prototype strain dove/Tennessee/4/75 was determined. The genome size is 15,480 nucleotides (nt) long and follows the "rule of six". The genome contains six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5'.
Catarina Guimarães-Teixeira et al.
Journal of personalized medicine, 11(10) (2021-10-24)
(1) Background: Methylation of N6-adenosine (m6A) is the most abundant messenger RNA (mRNA) modification in eukaryotes. We assessed the expression profiles of m6A regulatory proteins in renal cell carcinoma (RCC) and their clinical relevance, namely, as potential biomarkers. (2) Methods:
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Global Trade Item Number

SKUGTIN
WTA1-50RXN04061835378807