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X100RS

Sigma-Aldrich

Triton X-100 reduced

Synonym(s):
Polyoxyethylene (10) isooctylcyclohexyl ether
Linear Formula:
4-(C8H17)C6H10(OCH2CH2)nOH, n~10
CAS Number:
MDL number:
NACRES:
NA.25

description

non-ionic

Quality Level

mol wt

micellar average mol wt 80,000
average mol wt 625

aggregation number

100-155

refractive index

n20/D 1.473 (lit.)

CMC

0.2-0.9

transition temp

cloud point 65 °C

density

1.029 g/mL at 25 °C (lit.)

absorption

≤0.25 at 277 nm in H2O at 0.5%

HLB

13.5

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General description

Triton X-100 reduced, is a nonionic surfactant which has a hydrophilic polyethylene oxide chain.

Application

Triton X-100 reduced, a nonionic surfactant which has a hydrophilic polyethylene oxide chain, is a common laboratory detergent used for applications such as cell lysis and membrane permeabilization as a component of lysis buffers. Triton X-100 is used for the isolation of lipid rafts.

Packaging

5, 25 g in glass bottle

Preparation Note

Hydrogenated to reduce UV absorbance.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

235.4 °F - closed cup

Flash Point(C)

113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Mammalian long-chain acyl-CoA synthetases.
Soupene E, Kuypers FA.
Exp. Biol. Med, 233, 507-521 (2008)
Ki-Bum Kim et al.
Methods in molecular biology (Clifton, N.J.), 424, 413-422 (2008-03-29)
Because lipid rafts are plasma membrane platforms mediating various cellular events such as in signal transduction, immunological response, pathogen invasion, and neurodegenerative diseases, protein identification in the rafts could provide important information to study their function. Here, we present an...
Xin Ge et al.
Neuroreport, 28(10), 571-578 (2017-06-01)
One of the widely accepted hypotheses of Hirschsprung's disease (HD) is that the absence of ganglion cells in the distal part of the intestine is caused by the death of enteric neural crest-derived cells following migration. Although a caspase-dependent pathway...
Luke H Chamberlain
FEBS letters, 559(1-3), 1-5 (2004-02-28)
The relative insolubility of lipid rafts in cold non-ionic detergents is the most widely used method to purify these fascinating membrane domains from intact cells or membranes. Most of what we know about lipid raft function has been derived from...
J Fernandez et al.
Analytical biochemistry, 201(2), 255-264 (1992-03-01)
A procedure for the generation and isolation of internal peptide fragments for less than 10 micrograms of protein bound to either polyvinylidene difluoride (PVDF) or nitrocellulose membranes after electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) is presented. This technique...

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