MilliporeSigma
All Photos(1)

X1875

Sigma-Aldrich

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

Synonym(s):
XOD, Xanthine:oxygen oxidoreductase
CAS Number:
Enzyme Commission number:
1.17.3.2.
EC Number:
MDL number:
NACRES:
NA.54

type

Grade I

Quality Level

form

ammonium sulfate suspension

specific activity

≥0.4 units/mg protein

foreign activity

uricase ≤0.5%

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8.[1] This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.[2]
Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used:
  • in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay[3]
  • in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity[4]
  • as a standard to determine XO activity[5]
  • as a standard to test the synergistic effect of docosahexaenoic acid (DHA)[6]

Packaging

5, 25 units in serum bottle

Biochem/physiol Actions

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.[2]
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c. [7]

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

Analysis Note

Protein determined by biuret

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Technical Information

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service