This enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL, yielding a clear solution. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/134/883/x4376pis.pdf
X4376
Xanthine Oxidase from bovine milk
lyophilized powder, 0.4-1.0 units/mg protein
Synonym(s):
XOD, Xanthine:oxygen oxidoreductase
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5 UNITS
$179.00
25 UNITS
$607.00
50 UNITS
$1,180.00
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5 UNITS
$179.00
25 UNITS
$607.00
50 UNITS
$1,180.00
About This Item
CAS Number:
Enzyme Commission number:
1.17.3.2.
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
bovine milk
Quality Level
form
lyophilized powder
specific activity
0.4-1.0 units/mg protein
mol wt
283 kDa
composition
Protein, 10.0-25.0%
storage temp.
2-8°C
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General description
Formerly E.C. 1.1.3.22
Application
Biochem/physiol Actions
The isoflavanoid genistein was shown to inhibit xanthine oxidase at concentrations between 0.1-4.0 μM and then activate xanthine oxidase at concentrations higher than 5 μM. [4]
Xanthine Oxidase acts as a potential biomarker for oxidative stress. It is also implicated in tissue and vascular injuries such as liver damage, inflammatory diseases and chronic heart failure.[3]
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Physical form
Contains 0.5% sodium salicylate.
Analysis Note
Protein determined by biuret
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Good morning, I have a question how to use Xanthine Oxidase in the form lyophilized powder? In what form can it be used? Can it be converted into a liquid form? If so, what should be the solvent? Should I use water or buffer? If a buffer witch one?
1 answer-
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