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X4376

Sigma-Aldrich

Xanthine Oxidase from bovine milk

lyophilized powder, 0.4-1.0 units/mg protein

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Synonym(s):
XOD, Xanthine:oxygen oxidoreductase
CAS Number:
9002-17-9
Enzyme Commission number:
1.17.3.2.
EC Number:
232-657-6
MDL number:
MFCD00082145
NACRES:
NA.54

biological source

bovine milk

Quality Level

300

form

lyophilized powder

specific activity

0.4-1.0 units/mg protein

mol wt

283 kDa

composition

Protein, 10.0-25.0%

storage temp.

2-8°C

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This Item
X4875X1875X4500
Xanthine Oxidase from bovine milk lyophilized powder, 0.4-1.0 units/mg protein

Sigma-Aldrich

X4376

Xanthine Oxidase from bovine milk

Sigma-Aldrich

Sigma-Aldrich

X4875

Xanthine Oxidase from bovine milk

Sigma-Aldrich

Sigma-Aldrich

X1875

Xanthine Oxidase from bovine milk

Xanthine Oxidase from bovine milk Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

Sigma-Aldrich

X4500

Xanthine Oxidase from bovine milk

form

lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

specific activity

0.4-1.0 units/mg protein

specific activity

≥0.1 units/mg protein

specific activity

≥0.4 units/mg protein

specific activity

≥0.8 units/mg protein

mol wt

283 kDa

mol wt

-

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

-

General description

Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used as a source for superoxide generation. It has also been used as a model enzyme in the internally calibrated electrochemical continuous enzyme assay (ICECEA).

Biochem/physiol Actions

The isoflavanoid genistein was shown to inhibit xanthine oxidase at concentrations between 0.1-4.0 μM and then activate xanthine oxidase at concentrations higher than 5 μM.
Xanthine Oxidase acts as a potential biomarker for oxidative stress. It is also implicated in tissue and vascular injuries such as liver damage, inflammatory diseases and chronic heart failure.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Contains 0.5% sodium salicylate.

Analysis Note

Protein determined by biuret

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Massimiliano Magro et al.
Materials (Basel, Switzerland), 13(7) (2020-04-16)
Generally, enzyme immobilization on nanoparticles leads to nano-conjugates presenting partially preserved, or even absent, biological properties. Notwithstanding, recent research demonstrated that the coupling to nanomaterials can improve the activity of immobilized enzymes. Herein, xanthine oxidase (XO) was immobilized by self-assembly
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Rapid electrochemical enzyme assay with enzyme-free calibration
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