XNAP2E

Extract-N-Amp^™ Plant PCR Kit

sufficient for 100 extractions, sufficient for 500 amplifications

• Synonym(s): Plant direct PCR kit
• NACRES: NA.55

Properties

• usage: sufficient for 100 extractions; sufficient for 500 amplifications; sufficient for 500 reactions
• Quality Level: 200
• feature: dNTPs included; hotstart
• color: colorless
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The Extract-N-Amp^™ Plant PCR Kits intended for direct PCR (polymerase chain reaction) contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA.

Application

Extract-N-Amp^™ Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).[1][2][3][4][5]

Extract-N-Amp^™ Plant PCR Kit is suitable for use in genotyping.

Features and Benefits

• Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• Compatible with high-throughput requirements for genetic analysis of plants
• Convenient – no long enzymatic digestions
• Extract stable at 4 °C for at least 6 months

Components

Extract-N-Amp^™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable