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XNAPG
SYBR® Green Extract-N-Amp™ Plant PCR Kit
sufficient for 100 preparations
Recommended Products
usage
sufficient for 100 preparations
sufficient for 100 reactions
Quality Level
feature
dNTPs included
hotstart
technique(s)
qPCR: suitable
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
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This Item | XNAPS | XNATG | XNAS2 |
|---|---|---|---|
| usage sufficient for 100 preparations | usage sufficient for 10 amplifications, sufficient for 10 extractions, sufficient for 10 reactions | usage sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions | usage sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions |
| feature dNTPs included | feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
| detection method SYBR® Green | detection method - | detection method - | detection method - |
| shipped in wet ice | shipped in - | shipped in - | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
Features and Benefits
- Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- No loading buffers or tracking dyes required for gel analysis
- SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Compatible with high-throughput requirements for genetic analysis of plants
- No long enzymatic digestions
- Extract stable at 4°C for at least 6 months
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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