XNAT2R

Sigma-Aldrich

Extract-N-Amp Tissue PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

NACRES:
NA.55

Quality Level

usage

sufficient for 1000 amplifications
sufficient for 1000 extractions

feature

hotstart

color

colorless

shipped in

wet ice

storage temp.

−20°C

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General description

The Extract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR.

Application

Extract-N-Amp tissue PCR kit has been used for DNA extraction and polymerase chain reaction (PCR).

Features and Benefits

  • Perfect for quick genomic DNA isolation for genotyping
  • Single-step extraction of genomic DNA for PCR in 15 minutes
  • PCR ReadyMix is specially formulated for amplification directly from extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4 oC for at least 6 months

Components

Kits contain Tissue Preparation Solution, Extraction Solution, and Extract-N-Amp PCR Reaction Mix.

Principle

The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Target Organs

Respiratory system

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Sergio Y Alcoser et al.
BMC biotechnology, 11, 124-124 (2011-12-20)
Xenograft samples used to test anti-cancer drug efficacies and toxicities in vivo contain an unknown mix of mouse and human cells. Evaluation of drug activity can be confounded by samples containing large amounts of contaminating mouse tissue. We have developed...
Gergely Vida et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(12), 4476-4485 (2011-08-16)
The nervous system is classically organized into sympathetic and parasympathetic systems acting in opposition to maintain physiological homeostasis. Here, we report that both systems converge in the activation of β2-adrenoceptors of splenic regulatory lymphocytes to control systemic inflammation. Vagus nerve...
Chelsea D Mullins et al.
PloS one, 8(6), e67941-e67941 (2013-07-05)
Activating mutations in Ras (N- and K-) are the most common point mutations found in patients with multiple myeloma (MM) and are associated with poor clinical outcome. We sought to directly examine the role of Ras activation in MM pathogenesis...
Xiaodong Sun et al.
Cell cycle (Georgetown, Tex.), 12(7), 1133-1141 (2013-03-09)
Parkin, an E3 ubiquitin ligase well known for its role in the pathogenesis of juvenile Parkinson disease, has been considered as a candidate tumor suppressor in certain types of cancer. It remains unknown whether parkin is involved in the development...
Graziella Madeo et al.
Movement disorders : official journal of the Movement Disorder Society, 29(1), 41-53 (2013-10-30)
Homozygous or compound heterozygous mutations in the phosphatase and tensin homolog-induced putative kinase 1 (PINK1) gene are causative of autosomal recessive, early onset Parkinson's disease. Single heterozygous mutations have been detected repeatedly both in a subset of patients and in...
Articles
The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit
Read More
Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
Read More
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Read More
Protocols
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Read More

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