Y0626

Sigma-Aldrich

Yeast Nitrogen Base Without Amino Acids

Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

non-sterile

form

powder

application(s)

microbe id | utilization test: suitable

pH range

5.2 - 5.6 (0.67% solution)

Featured Industry

Food and Beverages

storage temp.

room temp

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts

Related Categories

General description

Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

Application

Yeast Nitrogen Base Without Amino Acids has been used:
  • in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterization
  • as a component of selective liquid media for uracil auxothrophic growth in yeast
  • to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Preparation Note

1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

T Fernandes et al.
Journal of applied microbiology, 127(3), 845-855 (2019-06-19)
The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial...
Simultaneous saccharification and fermentation of cellulose in ionic liquid for efficient production of alpha-ketoglutaric acid by Yarrowia lipolytica
Ryu S, et al.
Applied Microbiology and Biotechnology, 99(10), 4237-4244 (2015)
Activating and elucidating metabolism of complex sugars in Yarrowia lipolytica
Ryu S, et al.
Applied and Environmental Microbiology, 82(4), 1334-1345 (2016)
R Daniel Gietz et al.
Nature protocols, 2(1), 38-41 (2007-04-03)
Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes...
YAC Protocols, 11-11 (2006)
Articles
Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.
Read More
Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.
Read More
Protocols
Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.
Read More
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.
Read More

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