Y1375

YPD Broth

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): Yeast Peptone Dextrose Broth
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• composition: Bacteriological peptone, 20 g/L ; Glucose, 20 g/L ; Yeast extract, 10 g/L
• packaging: poly bottle of 1 kg; poly bottle of 250 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• color: beige
• pH: 6.3-6.7
• application(s): environmental; food and beverages; microbiology
• suitability: nonselective for Candida spp.; nonselective for Pichia spp.; nonselective for Saccharomyces spp.; nonselective for Zygosaccharomyces spp.

Description

General description

YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.

Application

YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.

Packaging

250 g in poly bottle

1 kg in poly bottle

Quantity

50 g per liter

Preparation Note

1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves