Y2146

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

• Synonym(s): Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements
• MDL number: MFCD01095679
• UNSPSC Code: 41106212
• NACRES: NA.85

Properties

• Quality Level: 200
• form: powder
• technique(s): transformation: suitable
• application(s): microbiology
• storage temp.: room temp

Description

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H318
• pcodes: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable