2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).
Y2377
2x YT medium
Powder microbial growth medium
Synonym(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
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grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.1% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
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This Item | Y2627 | Y1003 | T9179 |
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form powder | form liquid | form powder |
| sterility non-sterile | sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile |
| application(s) food and beverages | application(s) agriculture | application(s) microbiology | application(s) agriculture |
| pH 6.8-7.2(3.1% solution) | pH - | pH 6.5-7.5 | pH - |
General description
2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for culturing E. coli BL21 for cloning [1], E. coli SS320 cells for scaffold synthesis [2], and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens[3] and E. coli DH10B.[4]
Features and Benefits
2xYT powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Preparation Note
1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl
10 g/L Yeast Extract
5 g/L NaCl
related product
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Description
Pricing
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Bacterial artificial chromosomes: A functional genomics tool for the study of positive-strand RNA viruses
Yun SI, et al.
Journal of Visualized Experiments, 106, e53164-e53164 (2015)
Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants
Graham ND, et al.
Bio-protocol, 5(18) (2015)
Rebecca R Du et al.
ACS nano, 16(12), 20340-20352 (2022-12-03)
Three-dimensional wireframe DNA origami have programmable structural and sequence features that render them potentially suitable for prophylactic and therapeutic applications. However, their innate immunological properties, which stem from parameters including geometric shape and cytosine-phosphate-guanine dinucleotide (CpG) content, remain largely unknown.
Basem Al-Shayeb et al.
Cell, 185(24), 4574-4586 (2022-11-25)
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems. Bacteriophage-encoded
Designed active-site library reveals thousands of functional GFP variants
Weinstein J Y. et al.
Nature Communications (2023)
Protocols
General protocols for growth of competent cells in microbial medium.
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