Yeast Carbon Base

suitable for microbiology, NutriSelect® Basic


Quality Level






NutriSelect® Basic


microbe id | utilization test: suitable
microbiological culture: suitable
microbiology: suitable (microbial identification)

Featured Industry

Clinical Testing
Food and Beverages
Life Science and Biopharma

storage temp.



nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.


Recommended for use in classification of yeasts on the basis of their ability to assimilate nitrogen compounds.


Ingredients (g/L)
Dextrose, 10.00
L-Histidine hydrochloride, 0.001
DL-Methionine, 0.002
DL-Tryptophan, 0.002
Biotin, 0.000002
Calcium pantothenate, 0.0004
Folic acid, 0.000002
Inositol, 0.002
Niacin, 0.0004
p-Amino benzoic acid, 0.0002
Pyridoxine hydrochloride, 0.0004
Riboflavin (Vitamin B2), 0.0002
Thiamine hydrochloride, 0.0004
Boric acid, 0.0005
Coppers sulfate, 0.00004
Potassium iodide, 0.0001
Ferric chloride, 0.0002
Manganese sulfate, 0.0004
Sodium molybdate, 0.0002
Zinc sulfate, 0.0004
Monopotassium phosphate, 1.00
Magnesium sulfate, 0.50
Sodium chloride, 0.10
Calcium chloride, 0.10

Preparation Note

A. Nitrogen Assimilation test
Prepare the broth base at 10 x concentration by dissolving 11.7 grams of Yeast Carbon Base in 100 mL of distilled water. Add the appropriate nitrogen source. Warm if necessary to dissolve the medium completely. Sterilize by filtration.
B. Detection of yeasts, other than Saccharomyces cerevisae
Dissolve 2.35 grams of Yeast Carbon Base in 100 mL of distilled water.
C. Detection of wild yeasts in beer and other brewing components
Add 0.33 g of ammonium sulphate (Catalog No. A2939) and 4 g of bacteriological agar (Catalog No. A5306) to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.


We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.
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