Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

Z359629

Bright-Line™ Hemacytometer

Bright-Line™ Hemacytometer

supplied with two cover slips

Synonym(s):

hemacytometers

Sign Into View Organizational & Contract Pricing

Select a Size

1 EA
$273.00

$273.00

Available to ship onMay 13, 2025Details

All Photos(1)

Select a Size

Change View
1 EA
$273.00

About This Item

UNSPSC Code:
41122600
NACRES:
NB.22

$273.00

Available to ship onMay 13, 2025Details

material

colorless
glass

sterility

non-sterile

wells

2

Related Categories

General description

The Bright-Line™ Hemacytometer is molded from a single piece of thermal and shock-resistant glass. An H-shaped moat forms two counting areas, or plateaus. A "V" slash at the loading side of each plateau facilitates charing and reduces the possibility of overflow into the moat. Each plateau features enhanced Neubauer rulings.

The ruled surface is 0.1mm below the cover glass, limiting the volume of blood or fluid over a square mm at 0.1cu.mm and over each of 400 squares (within the central square mm) to 0.00025 cu. mm. Contact of the flat, polished cover glass surfaces with cover glass supports produces an exact volume of fluid over the counting area. The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.

Replacement cover slips sold separately: Z375357

Application

Bright-Line™ Hemacytometer is used for cell counting.[1] It may be used for determining the spore concentrations in spore suspension.[2] It might also be used to count the number of oocytes present in stock solutions.[3]

Legal Information

Bright-Line is a trademark of Cambridge Instruments, Inc.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

R Weber et al.
Journal of clinical microbiology, 29(7), 1323-1327 (1991-07-01)
To determine the minimum number of Cryptosporidium oocysts that can be detected in stool specimens by diagnostic procedures, stool samples seeded with known numbers of Cryptosporidium parvum oocysts were processed by the modified Formalin-ethyl acetate (FEA) stool concentration method. FEA
Francis A Armah et al.
Journal of ethnopharmacology, 211, 207-216 (2017-10-04)
Leishmaniasis is one of the neglected tropical disease caused by a protozoan of the genus Leishmania transmitted by sandflies. High cost and lack of oral formulation of existing drugs, rapid developments of resistance by the parasite coupled with serious side
Henry K Lin et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 16(20), 5011-5018 (2010-09-30)
Sensitive detection and characterization of circulating tumor cells (CTC) could revolutionize the approach to patients with early-stage and metastatic cancer. The current methodologies have significant limitations, including limited capture efficiency and ability to characterize captured cells. Here, we report the
Ogochukwu Izuegbuna et al.
PloS one, 14(1), e0209682-e0209682 (2019-01-30)
The Opuntia spp. have been used in traditional medicine for many centuries. It is used in the management of diseases that involves oxidative stress, especially diabetes, obesity and cancer. Opuntia stricta (Haw) is one of the relatively unknown species in
Rafał L Górny et al.
Applied and environmental microbiology, 68(7), 3522-3531 (2002-06-29)
The aerosolization process of fungal propagules of three species (Aspergillus versicolor, Penicillium melinii, and Cladosporium cladosporioides) was studied by using a newly designed and constructed aerosolization chamber. We discovered that fungal fragments are aerosolized simultaneously with spores from contaminated agar
View All Related Papers

Articles

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Protocols

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

1 Review This action will navigate to reviews.

Questions

1–6 of 6 Questions  

  1. Can the Z359629 Bright-Line™ Hemacytometer can be reused? Whether cover slip provided with the hemocytometer is reusable?

    1 answer

    1. Yes, the glass and the cover slip provided are re-usable.

      Helpful?

  2. Can the Z359629 Bright-Line™ Hemacytometer be used with a Phase Contrast Microscope?

    1 answer

    1. Yes, the Z359629 Bright-Line™ Hemacytometer can be used with a Phase Contrast Microscope.

      Helpful?

  3. Is this hemacytometer for use with phase microscopes?

    1 answer

    1. This product is not listed as suitable for phase microscopy and is not recommended. Please see the link below to review product HS3200, which is manufactured for use with phase microscopes:
      https://www.sigmaaldrich.com/product/hausserscientific/hs3200

      Helpful?

  4. How many coverslips they give along with the haemocytometer.

    1 answer

    1. As listed on the Product Detail Page, this product is supplied with two cover slips.

      Helpful?

  5. What is the ruling pattern used for Product Z359629, Bright-Line Hemacytometer?

    1 answer

    1. The details for Product Z359629, Bright-Line Hemacytometer are below: Cell Depth: 0.100mm +/- 2% (1/10mm)Volume: 0.1 MicroliterRuling Pattern: Improved Neubauer, 1/400 Square mmRulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service