Z4250

Zymosan A from Saccharomyces cerevisiae

for inducing inflamatory response

• Synonym(s): Yeast Cell Wall
• CAS Number: 58856-93-2
• MDL number: MFCD00082157

Properties

• biological source: Saccharomyces cerevisiae
• Quality Level: 200
• form: powder with small lumps
• technique(s): flow cytometry: suitable; tissue culture: suitable
• color: off-white to light brown
• storage temp.: 2-8°C

Description

Application

• in flow cytometry
• to study its effect on head kidney leukocytes from rainbow trout (Oncorhynchus mykiss)

Zymosan A from Saccharomyces cerevisiae has been used:

• as a positive control to determine the activation of complement cascade
• for respiratory burst (RB) assay
• to assess its effects on performance, immunity, heat shock protein and apoptosis related gene expressions of dairy cows under heat stress (HS)
• a stimulating agent in Krebs-Ringer phosphate (KRP) medium
• to induce thermal hyperalgesia in mice

Biochem/physiol Actions

Zymosan is prepared from yeast cell wall and consists of protein-carbohydrate complexes. It is used to induce experimental sterile inflammation. In macrophages, zymosan-induced responses include the induction of proinflammatory cytokines, arachidonate mobilization, protein phosphorylation, and inositol phosphate formation. Zymosan A also raises cyclin D2 levels suggesting a role for the latter in macrophage activation besides proliferation. It potentiates acute liver damage after galactosamine injection suggesting that certain types of nonparenchymal cells other than Kupffer cells are involved in zymosan action. Zymosan A, a 13 beta glucan contributes to the development of organic dust-induced respiratory disease.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves