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Whole Genome Amplification: Methods Express Series


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publication info

S. Hughes and R. Lasken, Scion Publishing, 2005, 300 pp., soft cover


Cold Spring Harbor

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General description

Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.

Table of Contents

2.Single nucleotide polymorphism typing using degenerated oligonucleotide primer-PCR amplified products as templates
3.WGA by improved primer extension preamplification (I-PEP)
4.Global Amplification using ′Scomp′
5.PRSG, a WGA method based on adaptor-ligation PCR of randomly sheared genomic DNA,
6.Omniplex WGA,
7.DNA Linear Amplification,
8.Multiple-displacement amplification (MDA) from clinical samples or extracted DNA,
9.MDA from single bacterial cells,
10.Genome amplification tolerant to sample degredation: application to FFPE specimens,
11.Preimplantation genetic diagnosis using WGA,

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Nazif Alic et al.
Molecular systems biology, 7, 502-502 (2011-06-23)
FoxO transcription factors, inhibited by insulin/insulin-like growth factor signalling (IIS), are crucial players in numerous organismal processes including lifespan. Using genomic tools, we uncover over 700 direct dFOXO targets in adult female Drosophila. dFOXO is directly required for transcription of

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