ThermalSeal RTS™ sealing film is a 50 μm thick polyolefin with 50 μm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert.
ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.
High optical clarity
Minimal to no autofluorescence
Chemically inert; no extractables except at extreme pH
DMSO resistant for HTS
Heat resistant, recommended for temperatures from
-70°C to +100°C
Certified DNase, RNase, and Nucleic Acid free
Fit within raised plate rim to prevent loss of seal due to film lifting
Silicone adhesive forms the strongest available seal for evaporation prevention
Non-tacky adhesive layer simplifies handling of film prior to sealing
ThermalSeal RTS is a trademark of Excel Scientific, Inc.
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
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