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ZRB1007

Sigma-Aldrich

Anti-Ki67 Antibody, clone 1O15, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Antigen KI-67, Antigen Ki67, Antigen identified by monoclonal antibody Ki-67, Proliferation marker protein Ki-67

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1O15, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 358.69 kDa
observed mol wt ~350 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400
western blot: suitable using 1:1,000

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MKI67(4288)

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species reactivity

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clone

1O15, recombinant monoclonal

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1O14, recombinant monoclonal

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Gene Information

human ... MKI67(4288)

Gene Information

-

Gene Information

-

Gene Information

-

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb® here.

Specificity

Clone 1O15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Proliferation marker protein Ki-67. It targets an epitope with in 14 amino acids from the C-terminal half.

Immunogen

KLH conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Proliferation marker protein Ki-67.

Application

Anti-Ki67, clone 1O15 ZooMAb, Cat. No ZRB1007, is a recombinant Rabbit Monoclonal Antibody that specifically targets Proliferation marker protein Ki-67 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ki67 in HUVEC, HeLa, and A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Ki67 in human tonsil and human skin tissue sections.

Target description

Proliferation marker protein Ki-67 (UniProt: P46013; also known as Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67) is encoded by the MKI67 gene (Gene ID: 4288) in human. Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle. Its expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen is undetectable. Ki67 is hyperphosphorylated in mitosis. Ki67 is required to maintain individual mitotic chromosomes and it disperses in the cytoplasm following nuclear envelope disassembly. It is shown to associate with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. It prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier. Two isoforms of Ki67 have been described that are produced by alternative splicing. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Xing Liang et al.
BMC gastroenterology, 23(1), 2-2 (2023-01-05)
Emerging evidence has shown the crucial roles of pleomorphic adenoma gene (PLAG) family genes in multiple cancers. However, their functions and mechanisms in pancreatic adenocarcinoma (PAAD) remain poorly understood. We analyzed the expression levels of PLAG family genes in both
Ruimeng Yang et al.
EBioMedicine, 63, 103195-103195 (2021-01-09)
Pancreatic cancer (PC) is one of the most lethal solid malignancies in the world due to its excessive cell proliferation and aggressive metastatic features. Emerging evidences revealed the importance of posttranscriptional modifications of RNAs in PC progression. However, knowledge about the 5-methylcytosine
Ratklao Siriwach et al.
iScience, 25(4), 104130-104130 (2022-04-09)
Keratinocyte differentiation is an intricate process that is regulated by multiple mediators. Using cultured human keratinocytes, we found that lysophosphatidic acid (LPA) induced the differentiation of a previously unsuspected keratinocyte subpopulation expressing the extracellular matrix protein, thrombospondin-1 (THBS1). This action
Zhi Teng et al.
Oncology reports, 43(2), 503-515 (2020-01-03)
Acute lymphoblastic leukemia (ALL) is a malignant hematological disease. Tanshinone IIA (Tan IIA) has antitumor activity in vitro and in vivo. The aim of the present study was to investigate the effects of Tan IIA in combination with imatinib (IM) on the proliferation, apoptosis, migration

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