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Anti-Choline Acetyltransferase (ChAT) Antibody, clone 2K16, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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CHOACTase, ChAT, Choline acetylase, EC:

biological source

rabbit (recombinant)

Quality Level


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


2K16, recombinant monoclonal

product line

ZooMAb® learn more



mol wt

calculated mol wt 82.54 kDa
observed mol wt ~65 kDa

species reactivity

rat, mouse

species reactivity (predicted by homology)



antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


immunofluorescence: suitable using 1:100
immunohistochemistry: suitable using 1:100
western blot: suitable using 1:1,000



UniProt accession no.

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... CHAT(1103)

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expressed in HEK 293 cells


expressed in HEK 293 cells


expressed in HEK 293 cells


expressed in HEK 293 cells









antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody


2K16, recombinant monoclonal


1B18, monoclonal, recombinant monoclonal


1K4, recombinant monoclonal


1L2, monoclonal, recombinant monoclonal

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 2K16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Choline Acetyltransferase. It targets an epitope with in 19 amino acids from the N-terminal half.


KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Choline acetyltransferase.


Anti-Choline Acetyltransferase (ChAT), clone 2K16 ZooMAb, Cat. No. ZRB1012, is a recombinant Rabbit Monoclonal Antibody that specifically targets Choline Acetyltransferase (aka ChAT) and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in rat brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue sections.

Target description

Choline O-acetyltransferase (UniProt: P28329; also known as EC:, CHOACTase, ChAT, Choline acetylase) is encoded by the CHAT gene (Gene ID: 1103) in human. Choline acetyltransferase a single-strand globular protein that catalyzes the biosynthesis of acetylcholine from acetyl CoA and choline. It is one of the most specific indicator for monitoring the functional state of cholinergic neurons in the central and peripheral nervous systems. It is synthesized in the perikaryon of cholinergic neurons and transported to the nerve terminals. Mutations in CHAT gene are known to cause Myasthenic syndrome that is characterized by failure of neuromuscular transmission, which include pre-synaptic, synaptic, and post-synaptic disorders. Three isoforms of Choline acetyltransferase have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oda, Y et al., (1999). Pathol. Int. 49(11); 921-937).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Jekaterina Zyuzin et al.
Journal of neurophysiology, 127(6), 1511-1518 (2022-04-21)
Pulmonary vagal nociceptors defend the airways. Cardiopulmonary vagal nociceptors synapse in the nucleus tractus solitarius (NTS). Evidence has demonstrated the convergence of cardiopulmonary nociceptors with afferents from carotid chemoreceptors. Whether sensory convergence occurs in motor nuclei and how sensory convergence
MiR34 contributes to spinal muscular atrophy and AAV9-mediated delivery of MiR34a ameliorates the motor deficits in SMA mice.
Chen, et al.
Molecular Therapy. Nucleic Acids, 32, 144-160 (2023)

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