ZRB1020

Sigma-Aldrich

Anti-Selenoprotein P Antibody, clone 2G23 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Quality Level

biological source

rabbit

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

2G23, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 43.17 kDa
observed mol wt ~53 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal half

conjugate

unconjugated

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2G23 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Selenoprotein P. It targets an epitope within 23 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human Selenoprotein P.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Selenoprotein P in HeLa cell lysate.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Selenoprotein P in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Selenoprotein P in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound Selenoprotein P peptide with a KD of 3.158 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Selenoprotein P, clone 2G23 ZooMAb®, Cat. No. ZRB1020, is a Rabbit monoclonal antibody that target Selenoprotein P and is tested for use in Affinity Binding Assay, Immunocytochemistry and Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Selenoprotein P (UniProt: P49908; also known as SeP) is encoded by the SELENOP (also known as SELP, SEPP1) gene (Gene ID: 6414) in human. Selenoprotein P is a secreted glycoprotein that is synthesized mainly in the liver and secreted into the plasma. It contains most of the selenium in plasma. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Secreted form of selenoprotein P is made up of two domains. The larger N-terminal domain contains one selenocysteine (U) in a U-x-x-C redox motif and the shorter C-terminal domain contains nine selenocysteines. It is shown to bind to heparin in a pH-sensitive manner at the heparin-binding site in its N-terminal region that contains two histidine residues that become protonated, and therefore can bind heparin. Selenoproteins provide a key defense against oxidative stress, as many of these proteins participate in oxidation-reduction reactions neutralizing reactive oxygen species, where selenocysteine residues act as catalytic sites. Selenoprotein P is removed from plasma by glomerular filtration by endocytosis mediated by LRP2 in the proximal tubule epithelium. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Burk, RF., and Hill, KE. (2009). Biochim. Biophys. Acta 1790(11); 1441-1447).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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