ZRB1067

Sigma-Aldrich

Anti-CDC5L Antibody, clone 4F8 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
Pombe cdc5-related protein, Cell division cycle 5-like protein, Cdc5-like protein

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F8, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 92.25 kDa
observed mol wt 110 kDa

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

conjugate

unconjugated

greener alternative category

Aligned

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4F8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cell division cycle 5-like protein (CDC5L). It targets an epitope within 25 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human cell division cycle-5 like protein (CDC5L).

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CDC5L in MCF-7, HeLa, U2OS, and NIH3T3 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CDC5L in HeLa cells and A431 cells.

Affinity Binding Assay: A representative lot of this antibody bound CDC5L peptide with a KD of 2.9 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CDC5L in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CDC5L, clone 4F8 ZooMAb, Cat. No. ZRB1067, is a Rabbit monoclonal antibody that specifically targets CDC5L and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Cell division cycle 5-like protein (UniProt: Q99459; also known as Cdc5-like protein, Pombe cdc5-related protein, CDC5L) is encoded by the CDC5L (also known as KIAA0432, PCDC5RP0) gene (Gene ID: 988) in human. CDC5L is a homodimeric DNA-binding protein that is involved in cell cycle control and may act as a transcription activator. It is shown to be a positive regulator of cell cycle G2/M progression. It is present in both the nucleus and the cytoplasm and shuttles between these two compartments. Its nuclear localization signal sequence is contained in amino acids 165-271. It is a core component of the human Prp19/CDC5L complex that is required for pre-mRNA processing. CDC5L also modulates the expression of a set of genes that are involved in the mitosis and the DNA damage response. Its phosphorylation on threonine 411 and threonine 438 is required for CDC5L-mediated mRNA splicing. CDC5L is shown to be highly expressed in cervical tumors and osteosarcoma and a chromosomal aberration involving CDC5l has been reported in multicystic renal dysplasia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mu, R., et al. (2014). Cell Death & Disease 5: e1151).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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