ZRB1153
Anti-53BP1 Antibody, clone 1H16 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
TP53-binding protein 1, p53-binding protein 1, p53BP1
About This Item
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
clone
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 213.57 kDa
observed mol wt ~270 kDa
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
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technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
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Specificity
Immunogen
Application
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 53BP1 in HEK293 cell nuclear extract.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected 53BP1 in HeLa cell Nuclear Extract.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected 53BP1 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound recombinant 53BP1 protein fragment with a KD of 1.2 x 10-8 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected 53BP1 in HeLa, A431, HepG2, NIH 3T3 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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