ZRB1167

Anti-Collagen Type III Antibody, clone 3N18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3N18, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 138.56 kDa; observed mol wt ~200 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_000081.1?
• UniProt accession no.: P02461
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Collagen alpha-1(III) chain (UniProt: P02461; also known as Collagen type IIIa) is encoded by the COL3A1 gene (Gene ID: 1281) in human. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagen with at least 38 distinct polypeptide chains. Collagen type III occurs in most soft connective tissues along with type I collagen. It is a major structural component in hollow organs such as large blood vessels and uterus that must withstand stretching. Collagen type III is also involved in regulation of cortical development. It is reported to be the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. It is synthesized with a signal peptide (aa 1-23), an N-terminal propeptide (aa 24-153), and a C-terminal propeptide (aa 1222-1466), which are subsequently cleaved off to generate the mature form. Three identical type III procollagen chains come together in the C-terminal ends to form collagen III molecule and it is stabilized by the formation of disulfide bonds. Each individual chain folds into left-handed helix and the three chains are then wrapped together into a right-handed superhelix, the triple helix. Mutations in COL3A1 are linked to Ehlers-Danlos syndrome, a connective tissue disorder that is characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kuivbaniemi, H., and Tromp, G. (2019). Gene. 707; 151-171).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Collagen a1(III) chain.

Application

Quality Control Testing

Evaluated by Western Blotting in HT1080 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Collagen Type III in HT1080 cell lysate.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collagen Type III in human skin tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Collagen Type III with a KD of 6.7 x 10-6 in an affinity binding assay.

ELISA Analysis: 300 μg/mL from a representative lot detected Collagen Type III in Collagen Type III in an indirect ELISA.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Collagen Type III, clone 3N18 ZooMAb^®, Cat. No. ZRB1167, is a recombinant Rabbit monoclonal antibody that detects Collagen Type III and is tested for use in Affinity Binding Assay, ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Biochem/physiol Actions

Clone 3N18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Collagen Type III. It targets an epitope with 22 amino acids from the C-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable