ZRB1297

Sigma-Aldrich

Anti-Endoglin Antibody, clone 1I15 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Quality Level

biological source

rabbit

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

1I15, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 70.58 and 67.54 kDa
observed mol wt ~90 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

conjugate

unconjugated

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1I15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human Endoglin (CD105). It targets an epitope within 17 amino acids from the extracellular domain in the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the extracellular domain in the C-terminal region of human Endoglin.

Application

Anti-Endoglin, clone 1I15 ZooMAb®, Cat. No. ZRB1297, is a recombinant Rabbit monoclonal antibody that specifically targets Endoglin and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in Human Lung tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Endoglin in Human Lung tissue lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Endoglin in Human umbilical vein endothelial cell (HUVEC) lysate.

Affinity Binding Assay: A representative lot of this antibody bound Endoglin Peptide with a KD of 3.8 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Endoglin in human kidney tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected Endoglin in one million Human umbilical vein endothelial cells (HUVEC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Endoglin (UniProt: P17813; also known as CD105) is encoded by the ENG (also known as END) gene (Gene ID: 2022) in human. Endoglin is a single-pass type I, disulfide-linked, membrane glycoprotein that is detected on placenta and umbilical vein endothelial cells. It can also form heterodimeric complex with the signaling receptors for TGFb. It is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 26-586), a transmembrane domain (aa 587-611), and a cytoplasmic domain (aa 612-658). Endoglin also contains a zona pellucida (ZP) domain (aa 363-533) that mediates its dimerization. Endoglin is reported to play an important role in the regulation of angiogenesis. It is also required for normal structure and integrity of adult vasculature. It regulates the migration of vascular endothelial cells and regulates the change in shape of endothelial cells in response to blood flow thereby causing vascular remodeling and establishment of normal vascular morphology during angiogenesis. It has been also reported to play a role in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin also serves as a co-receptor for TGFb and is involved in the TGFb/BMP signaling cascade leading to the activation of SMAD transcription factors. Mutations in ENG gene have been linked to hereditary hemorrhagic telangiectasia that is characterized by recurrent epistaxis and gastro-intestinal hemorrhage. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.