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ZRB1315

Sigma-Aldrich

Anti-VDAC1 Antibody, clone 3M22, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC-1, Voltage-dependent anion-selective channel protein 1 1, hVDAC1

biological source

rabbit (recombinant)

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3M22, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 30.77 kDa
observed mol wt ~35 kDa

species reactivity

rat, mouse, human

packaging

pkg of 25 μL

greener alternative product characteristics

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

mouse ... Vdac1(22333)

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 3M22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Voltage-dependent anion-selective channel protein 1 (VDAC1). It targets an epitope within the C-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal half of human Voltage-dependent anion-selective channel protein 1 (VDAC1).

Application

Anti-VDAC1, clone 3M22 ZooMAb, Cat. No. ZRB1315, is a recombinant rabbit monoclonal antibody that detects Voltage-dependent anion-selective channel protein 1 (VDAC1) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected VDAC1 in HEK293, NIH3T3, and C6 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VDAC1 in HeLa, A431, HUVEC, NIH 3T3, MCF-7, NTERA-2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VDAC1 in human kidney and human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Voltage-dependent anion-selective channel protein 1 (UniProt: P21796; also known as VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM) is encoded by the VDAC1 (also known as VDAC) gene (Gene ID: 7416) in human. VDAC1 is a multi-pass membrane protein that is expressed on the outer mitochondrial membrane (OMM). It is one of the most abundant proteins on OMM. It serves as the gatekeeper for the passages of metabolites, nucleotides, and ions and with its interaction with Bcl-2 it plays a crucial role in regulating apoptosis. VDAC1 consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. Its helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. It also participates in the formation of the permeability transition pore complex that is responsible for the release of mitochondrial products that triggers apoptosis. VDAC1 adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is shown to be cation-selective and can transport K+, Na+ and Ca2+. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Camara, AKS et al. (2017). Front. Physiol. 8; 460).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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