ZRB1520
Anti-phospho-Stat1-Tyr701 Antibody, clone 1F23 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1F23, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 87.33 kDa
observed mol wt ~91 kDa
species reactivity
human
species reactivity (predicted by homology)
bovine, monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pTyr701)
Gene Information
human ... STAT1(6772)
General description
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Immunogen
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Stat1-Tyr701 in HeLa cells, serum starved and treated with interferon a (2000 Units/mL; 20 min.).
Peptide Inhibition Assay: Target band detection in 10 μg of lysate from HeLa cells treated with interferon a was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Affinity Binding Assay: A representative lot of this antibody bound phosphorylated Stat1-Tyr701 peptide with a KD of 7.9 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
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