The solution stability of NTA - Atto 647 N has not been evaluated. The original Atto 647 N is not stable in aqueous solutions for long-term storage and should be prepared fresh. Solutions prepared in anhydrous, amine-free DMSO or Dimethylformamide may offer some benefit to solution stability. It would be up to the end user to determine long-term storage, however, general practice is typically to aliquot and store at -20 °C, avoiding repeated freeze/thaw cycles.
02175
NTA - Atto 647 N
BioReagent, suitable for fluorescence, ≥80% (HPCE)
Synonym(s):
Atto 647 N - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
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About This Item
Recommended Products
product line
BioReagent
Quality Level
assay
≥80% (HPCE)
fluorescence
λex 647 nm; λem 669 nm in PBS
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
General description
Application
Legal Information
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hi! What is the recommendations for storing of dissolved in PBS Ni-NTA-atto-647? Can I do multiple freeze/thaw cycles?
1 answer-
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What is the molecular weight of this product ?
1 answer-
The molecular weight of this product is 1120 g/mol.
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How is Product 02175, NTA-Atto 647N, to be dissolved and diluted?
1 answer-
The 250 μg of 02175-250UG-F, NTA-Atto 647N, may be dissolved in 250 μL of phosphate buffered saline (PBS), pH 7.4, to make a 1 mg/mL stock solution. This stock solution may be diluted by a factor of 1:1000 as follows:---For use on gel: 25 μL of stock in 25 mL PBS.---For use on Western Blot (PVDF membrane): 25 μL of stock in 25 mL PBST (PBS plus 0.1% TWEEN 20).
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