Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

02175

Supelco

NTA - Atto 647 N

BioReagent, suitable for fluorescence, ≥80% (HPCE)

Synonym(s):

Atto 647 N - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex

Sign Into View Organizational & Contract Pricing

Select a Size

250 μG
$403.00

$403.00


In StockDetails


Request a Bulk Order

Select a Size

Change View
250 μG
$403.00

About This Item

UNSPSC Code:
12171501
NACRES:
NA.32

$403.00


In StockDetails


Request a Bulk Order

product line

BioReagent

Quality Level

assay

≥80% (HPCE)

fluorescence

λex 647 nm; λem 669 nm in PBS

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

General description

find more information here

Application

for the fluorescence detection of HIS-tagged proteins

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

pictograms

Health hazardEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–3 of 3 Questions  
  1. Hi! What is the recommendations for storing of dissolved in PBS Ni-NTA-atto-647? Can I do multiple freeze/thaw cycles?

    1 answer
    1. The solution stability of NTA - Atto 647 N has not been evaluated. The original Atto 647 N is not stable in aqueous solutions for long-term storage and should be prepared fresh. Solutions prepared in anhydrous, amine-free DMSO or Dimethylformamide may offer some benefit to solution stability. It would be up to the end user to determine long-term storage, however, general practice is typically to aliquot and store at -20 °C, avoiding repeated freeze/thaw cycles.

      Helpful?

  2. What is the molecular weight of this product ? 

    1 answer
    1. The molecular weight of this product is 1120 g/mol.

      Helpful?

  3. How is Product 02175, NTA-Atto 647N, to be dissolved and diluted?

    1 answer
    1. The 250 μg of 02175-250UG-F, NTA-Atto 647N, may be dissolved in 250 μL of phosphate buffered saline (PBS), pH 7.4, to make a 1 mg/mL stock solution. This stock solution may be diluted by a factor of 1:1000 as follows:---For use on gel: 25 μL of stock in 25 mL PBS.---For use on Western Blot (PVDF membrane): 25 μL of stock in 25 mL PBST (PBS plus 0.1% TWEEN 20).

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service