Astec CHIROBIOTIC™ TAG columns showing decreased resolution sometimes can be regenerated by passing several column volumes of a 50/50 solution of acetonitrile and 50 mM ammonium acetate (NH4OAc) aqueous buffer. This is followed by passing several column volumes of pure HPLC grade water, and then by passing several column volumes of acetonitrile or methanol, all at a flow rate of 0.5 mL/min. Acetonitrile or methanol may be used for final displacement and storage. For long term storage (>24 hours) isopropanol or methanol is recommended.
14024AST
Astec® CHIROBIOTIC® TAG Chiral (5 μm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
teicoplanin aglycone phase
Pore size:
100 Å
Matrix:
High-purity silica gel particle platform, fully porous particle
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Product Name
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L63
description
HPLC column
product line
Astec®
packaging
pkg of 1 ea
manufacturer/tradename
Astec®
parameter
0-45 °C temperature, 241 bar pressure (3500 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
matrix
High-purity silica gel particle platform, fully porous particle
matrix active group
teicoplanin aglycone phase
particle size
5 μm
pore size
100 Å
operating pH range
3.0-6.8
separation technique
chiral
Quality Level
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Related Categories
1 of 4
This Item | 14018AST | 14019AST | 14020AST |
|---|---|---|---|
| particle size 5 μm | particle size 5 μm | particle size 5 μm | particle size 5 μm |
| separation technique chiral | separation technique chiral | separation technique chiral | separation technique chiral |
| matrix active group teicoplanin aglycone phase | matrix active group teicoplanin aglycone phase | matrix active group teicoplanin aglycone phase | matrix active group teicoplanin aglycone phase |
| L × I.D. 25 cm × 4.6 mm | L × I.D. 10 cm × 2.1 mm | L × I.D. 15 cm × 2.1 mm | L × I.D. 25 cm × 2.1 mm |
| agency suitable for USP L63 | agency suitable for USP L63 | agency suitable for USP L63 | agency suitable for USP L63 |
| pore size 100 Å | pore size 100 Å | pore size 100 Å | pore size 100 Å |
Application
- High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.: This study emphasizes the effectiveness of the Astec® CHIROBIOTIC® TAG Chiral HPLC Column in the enantioseparation of complex cyclic amino acids. The methodology detailed provides profound insights into the chirality aspects of biochemical molecules, which is crucial for the synthesis of novel pharmaceutical agents and the study of biochemical pathways. This research supports analytical chemists in academia focused on developing chiral analytical methods and advancing chemical synthesis (Berkecz et al., 2009).
General description
The removal of three carbohydrate moieties gives CHIROBIOTIC® TAG complementary selectivity to CHIROBIOTIC® T. Resolution is enhanced toward many of the amino acids, α, β, γ and cyclic, and especially sulfur-containing methionine, histidine and cysteine. A number of neutral molecules like the oxazolidinones, hydantoins and diazepines have shown enhanced resolution and, more remarkably, in the single solvents like methanol, ethanol and acetonitrile. Some acidic molecules have also shown increased selectivity.
- Bonded phase: Teicoplanin aglycone
- Operating pH range: 3.0 - 6.8
- Particle diameter: 5, 10 or 16 μm
- Pore size: 100 Å
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Markus Fuereder et al.
Journal of chromatography. A, 1236, 123-131 (2012-04-03)
Teicoplanin aglycone-based chromatography columns (Chirobiotic TAG) enable amino acid enantioseparation with aqueous mobile phases, which perfectly accommodates the distinct hydrophilicity of most amino acids. Therefore, this stationary phase constitutes a promising option in particular for preparative-scale separations that require high
Abdulrahman A Al-Majed
Journal of pharmaceutical and biomedical analysis, 50(1), 96-99 (2009-05-19)
A direct chiral high-performance liquid chromatography (HPLC) method was developed and validated for the resolution and quantification of antiepileptic drug enantiomers, R-(-)- and S-(+)-vigabatrin (gamma-vinyl-gamma-aminobutyric acid) in pharmaceutical products. The separation was optimized on a macrocyclic glycopeptide antibiotic chiral stationary
Ilaria D'Acquarica et al.
Journal of chromatography. A, 1061(2), 167-173 (2005-01-12)
We describe a new tandem-columns chiral-achiral HPLC arrangement by using a chiral column (CHIROBIOTIC TAG) connected in series with an achiral column (Spherisorb S5 SCX), based on a strong cationic exchange mechanism; this approach is very useful for the analysis
Adsorption behavior of a teicoplanin aglycone bonded stationary phase under harsh overload conditions
Bechtold, Matthias, et al.
Journal of Chromatography A, 1154 (1-2), 2477-2286 (2007)
Determination of eflornithine enantiomers in plasma, by solid-phase extraction and liquid chromatography with evaporative light-scattering detection
Malm, M., Bergqvist, Y.
Journal of Chromatography. B, Biomedical Sciences and Applications, 846 (1-2), 98-104 (2007)
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which is suitable solvent mixture for the cleaning of Astec chirobitic TAG columns (Cat. No. 14024AST)? What is re-generation procedure for this column?
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