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21568-U

Supelco

Supelite DAX-8

jar of 5000 g

Synonym(s):
XAD-8
NACRES:
SB.52

form

solid

Quality Level

packaging

jar of 5000 g

technique(s)

HPLC: suitable
LPLC: suitable
ion chromatography: suitable

matrix

acrylic ester

matrix active group

polymer

particle size

40-60 mesh

pore size

~0.79 mL/g pore volume
225 Å mean pore size

surface area

140 m2/g

density

1.09 g/mL at 25 °C (true wet)(lit.)
1.23 g/mL at 25 °C (skeletal)(lit.)

separation technique

reversed phase

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General description

Supelite DAX-8 is a nonfunctionalised resin with strong hydrophobic organic matter endorsed to humic and fulvic acids. It is also referred as polymethylmethacrylate resin.

Application

Supelite DAX-8 was used as an absorbent in:
  • HPLC-SEC fractionation system
  • column chromatography
  • extraction procedure and these extracts were analysed using Cary Eclipse fluorescence spectrophotometer
Adsorbent resin with moderate polarity for compounds up to 150,000 MW. Most commonly used for adsorption of fulvic and humic acids, treatment of paper pulp mill waste, and removal of alcohols, surfactants, and colorants from samples.

Preparation Note

DAX-8 is a salt imbibed resin, which requires some preparation prior to use. In the protocol below, you may use methanol in place of ethanol.
  • First, rinse the adsorbent resin with 2-3 bed volumes (BV) of distilled water.
  • Soak overnight in 2 BV of 95% ethanol.
  • After soaking, the resin is introduced into the column and rinsed with an additional 2 BV of 95% ethanol.
  • Rinse with 2 BV of distilled water to displace the ethanol, followed by 1 BV of 4% (w/v) sodium hydroxide, 2 BV of distilled water, 1 BV 4% (v/v) hydrochloric acid, and finally by distilled water until the pH of the eluent becomes neutral.

Legal Information

Supelite is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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