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24308

Supelco

β-DEX™ 325

L × I.D. 30 m × 0.25 mm, df 0.25 μm

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Synonym(s):
β-DEX™ 325, 30M .25MM .25UM
eCl@ss:
32119290

material

fused silica

description

GC capillary column

parameter

30-230 °C temperature

Beta value

250

df

0.25 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

30 m × 0.25 mm

matrix active group

non-bonded; 25% 2,3-di-O-methyl-6-O-TBDMS-β-cyclodextrin in SPB-20 poly(20% phenyl/80% dimethylsiloxane) phase

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
life science and biopharma
personal care
pharmaceutical (small molecule)

column type

capillary chiral

separation technique

chiral

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β-DEX™ 325

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γ-DEX™ 325

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α-DEX™ 225

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parameter

30-230 °C temperature

parameter

30-230 °C temperature

parameter

30-230 °C temperature

parameter

30-230 °C temperature

df

0.25 μm

df

0.25 μm

df

0.25 μm

df

0.25 μm

technique(s)

gas chromatography (GC): suitable

technique(s)

gas chromatography (GC): suitable

technique(s)

gas chromatography (GC): suitable

technique(s)

gas chromatography (GC): suitable

matrix active group

non-bonded; 25% 2,3-di-O-methyl-6-O-TBDMS-β-cyclodextrin in SPB-20 poly(20% phenyl/80% dimethylsiloxane) phase

matrix active group

non-bonded; 25% 2,3-di-O-methyl-6-O-TBDMS-γ-cyclodextrin in SPB-20 poly(20% phenyl/80% dimethylsiloxane) phase

matrix active group

non-bonded; 25% 2,3-di-O-acetyl-6-O-TBDMS-α-cyclodextrin in SPB-20 poly(20% phenyl/80% dimethylsiloxane) phase

matrix active group

non-bonded; 25% 2,3-di-O-acetyl-6-O-TBDMS-γ-cyclodextrin in SPB-20 poly(20% phenyl/80% dimethylsiloxane) phase

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
life science and biopharma
personal care
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
life science and biopharma
personal care
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
life science and biopharma
personal care
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
life science and biopharma
personal care
pharmaceutical (small molecule)

General description

The chiral stationary phase in β-DEX 325 columns contains 2,3-di-O-methyl-6-0-TBDMS-β-cyclodextrin embedded in an intermediate polarity phase. The Supelco β-DEX 325 is similar in both chemistry and use to the CHIRALDEX B-DM phase, the main difference being the concentration of the dimethyl-derivatized cyclodextrin that is doped into the polysiloxane carrier.

Application

ß-DEX™-325 column was used in gas-liquid chromatography (GLC), for determination of (R)-1-O-acetyl-2-ethyl-1,3-propanediol, formed from 2-Ethyl-1,3-propanediol 1 and its related di-O-acetate, by partial chemoenzymatic acetylation and deacetylation, by Pseudomonas fluorescens lipase. ß -Dex™-325 capillary column was also used for resolving 8-acetoxylinalool enantiomers, in an experimental study done in order to study the origin of the enantioselectivity in the biosynthesis of the lilac compounds in Actinidia arguta flowers.

Chem/Phys Resistance

Temp. Limits:
  • 30 °C to 230 °C

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

DEX is a trademark of Sigma-Aldrich Co. LLC

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A J Matich et al.
Phytochemistry, 68(13), 1746-1751 (2007-05-01)
Biosynthesis of lilac compounds in 'Hortgem Tahi' kiwifruit (Actinidia arguta) flowers was investigated by treating inflorescences with d(5)-linalool. The incorporation of the deuterium label into 8-hydroxylinalool, 8-oxolinalool, the lilac aldehydes, alcohols, and alcohol epoxides was followed by GC-MS and enantioselective
Chiral building-blocks by chemoenzymatic desymmetrization of 2-ethyl-1, 3-propanediol for the preparation of biologically active natural products.
Isidoro I
Tetrahedron Asymmetry, 10 (3), 449-455 (1999)

Chromatograms

suitable for GC

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