50137-U

LiChrospher^® RP-18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

• Synonym(s): TM=["LiChrospher"] C18 HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52
• L × i.d.: 25 cm × 4 mm
• Particle size: 5 μm
• Matrix active group: C18 (octadecyl) phase
• Pore size: 100 Å
• Matrix: octadecyl, silica particle platform, fully porous particle

Properties

• Product Name: LiChrospher^® RP-18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
• material: stainless steel column
• agency: suitable for USP L1
• product line: LiChrospher^®
• feature: endcapped: no
• manufacturer/tradename: LiChrospher^®
• packaging: 1 ea of
• extent of labeling: 21% Carbon loading
• parameter: ≤70 °C temp. range, 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 4 mm
• surface area: 350 m²/g
• matrix: octadecyl, silica particle platform, fully porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH: 2-7.5
• application(s): food and beverages
• separation technique: reversed phase
• Quality Level: 100

Description

General description

LiChrospher^® RP-18 HPLC column (L × I.D. 25 cm × 4 mm) is found to be reliable and high versatile. Suitable for reversed-phase high performance liquid chromatography (RP-HPLC) of acidic, neutral and weakly basic compounds, according to L1 USP specifications.

Application

• HPLC-UV and GC-MS Methods for Determination of Chlorambucil and Valproic Acid in Plasma for Further Exploring a New Combined Therapy of Chronic Lymphocytic Leukemia.: This study developed and validated HPLC-UV and GC-MS methods for determining chlorambucil and valproic acid in plasma, aiming to explore their combined therapy for chronic lymphocytic leukemia. The LiChrospher^® RP-18 HPLC Column was instrumental in achieving the separation and quantification (Lipska et al., 2021).


• Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma.: This research utilized the LiChrospher^® RP-18 HPLC Column for the rapid determination of metronidazole and its metabolite in murine blood plasma, highlighting its effectiveness in pharmacokinetic studies (Zemanová et al., 2022).


• Application of chemometric approach for development and validation of high performance liquid chromatography method for estimation of ropinirole hydrochloride.: This paper presents a chemometric approach to developing and validating an HPLC method for estimating ropinirole hydrochloride, utilizing the LiChrospher^® RP-18 column for precise and accurate results (Fatima et al., 2019).


• A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography.: The study developed a novel solid-phase extraction method for determining pantoprazole in human plasma using HPLC with the LiChrospher^® RP-18 column, achieving high sensitivity and specificity (Zendelovska et al., 2019).


• Application of an HPLC-MS/MS method for Teicoplanin drug substance and related impurities, part 2: Identity assignment of related impurities.: This research employed the LiChrospher^® RP-18 column in an HPLC-MS/MS method to identify and assign related impurities in teicoplanin drug substances, demonstrating the column′s capability in impurity profiling (Tengattini et al., 2019).

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable