MilliporeSigma
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50270-U

Supelco

Ascentis® Express HILIC, 5 μm HPLC Column

5 μm particle size, L × I.D. 15 cm × 3 mm

NACRES:
SB.52

material

stainless steel column

Quality Level

Agency

suitable for USP L3

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 3 mm

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

silica phase

particle size

5 μm

pore size

90 Å pore size

surface area

90 m2/g

operating pH

1-8

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

Ascentis Express 5 μm HILIC is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell around and an overall particle size of ~5-microns. The bare silica stationary phase of Ascentis Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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