Skip to Content
MilliporeSigma

Skip To

504971

Discovery® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 ea

Available to ship onJune 02, 2026fromMILWAUKEE

$1,170.00

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C18 (RP18), C18 (octadecyl) phase
Pore size:
180 Å
Matrix:
silica gel, high purity, spherical particle platform

$1,170.00


Available to ship onJune 02, 2026Details


Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L1

Quality Segment

feature

endcapped

manufacturer/tradename

Discovery®

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

300 m2/g

surface coverage

3 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform

matrix active group

C18 (RP18), C18 (octadecyl) phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Application

Discovery® C18 HPLC columns provide a classic reversed-phase selectivity and retention with excellent peak shape for all compounds featuring high stability and no-bleed properties for LC-MS applications. Typical applications are the separation of Aflatoxins, Aldehydes, Alkaloids, Antibiotics, Aromatic amines, Catecholamines, Esters, Lipids, Fatty acids, Flavonoids, Ketones, Nitrosamines, Nucleosides, Nucleotides, PCB, Peptides, Pesticides, Phenols, Phospholipids, Phthalates, Preservatives, Steroids, Sulfonamides, Sweeteners and Vitamins.

Features and Benefits

  • Excellent reproducibility
  • Exceptional peak shape for basic and acidic analytes
  • Stable, low-bleed LC-MS separations
  • Separation of peptides and small proteins
  • Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
50494750495550494721
separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

L × I.D.

25 cm × 4.6 mm

L × I.D.

5 cm × 4.6 mm

L × I.D.

15 cm × 4.6 mm

L × I.D.

5 cm × 2.1 mm

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix

silica gel, high purity, spherical particle platform

matrix

silica gel, high purity, spherical particle platform

matrix

silica gel, high purity, spherical particle platform

matrix

silica gel, high purity, spherical particle platform

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable


Still not finding the right product?

Explore all of our products under Discovery® C18 HPLC Column


Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. What is the shipping solvent of the column?

    1 answer
    1. The shipping solvent of the column is 66% methanol and 34% water.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters